lOM oA R c P S D| 30 87 312 9
Lab 1 Introduction to Science BIO250L
BIO250L+V2+Lab+1 Latest
Exam Complete Questions and
Answer 100% Verified Correct.
Microbiology (Trinity College)
Student Name:
Access Code (located on the underside of the lid of your lab kit):
Pre-Lab Questions
1. What constitutes personal protective equipment? When should personal protective equipment
be worn? Why is personal protective equipment important?
Personal protective equipment Include: safety goggles face protection equipmet, gloves,
and lab coat/apron. For feet safety, shoe covers are advised During the carrying out of
any experiments Personal protective equipment should is worn so as to prevent the
spread of microbes and actual infections.
2. Why are there increasing levels of biological containment procedures? What level of biological
containment will be necessary to complete the experiments in this manual?
There are increasing levels of biological containment procedures for several reasons,
The main reason is to guarantee safety in the microbiology lab. There is also need to
prevent Laboratory Acquired Infections as well as the need to protect the scientists
working with pathogenic organisms and viruses in the course of their research. By
having biological containment preocedures in place, society is kept safe from
contamination too
3. What document should you refer to if you have questions regarding the safety or disposal of a
chemical? Where can this document be located?
In regarding the safety or disposal l the material safety data (MSDS) sheet is the point of
, lOM oA R c P S D| 30 87 312 9
Lab 1 Introduction to Science BIO250L
reference The MSDS can be found on the manufacturer’s website or in a binder placed
at a central place in our case the SDS can be found on
https://esciencelabs.com/educators/msds
4. List five safety procedures that should always be adhered to when working with microorganisms.
a. Hands must be washed thoroughly when one is done working with
microorganisms. b.
b. B. No Eating, drinking, smoking chewing of gum, smoking , touching contact
lenses, applying cosmetics, handling and storing food for human consumption
can be done in the laboratory
c. Before the start of an experiment all persons must read the instructions carefully.
d. Put on gloves before handling any specimen. Put on other PPE as needed,
people must wear closed shoes while in the lab
e. Decontaminate working surfaces after an experiment. Wash hands thoroughly
under running water
EXERCISE 1: DATA INTERPRETATION
1. What patterns do you observe based on the information in Table 4?
The more acidic the medium, the less microbes there are. As the PH approaches
neutral, the number of microbes increases and peaks at the ph of 7-Neutral. This trend
changes as soon as the ph starts to hit the alkalinic PH and number of microbes
continues to dwindle as the ph becomes more and more alkalinic.
2. Develop a hypothesis relating to the pH level of the culture media and the number of microbial
colonies observed in each culture.
Microbial activity increases and peaks as the ph approaches a ph of 7
3. What would your experimental approach be to test this hypothesis?
Set up a microbial culture and grow it under varying ph levels raning from most basic to
most acidic
4. What would be the independent and dependent variables?
The PH wold be the independent variable.
5. What would be your control?
A neutral ph would be the control
Lab 1 Introduction to Science BIO250L
BIO250L+V2+Lab+1 Latest
Exam Complete Questions and
Answer 100% Verified Correct.
Microbiology (Trinity College)
Student Name:
Access Code (located on the underside of the lid of your lab kit):
Pre-Lab Questions
1. What constitutes personal protective equipment? When should personal protective equipment
be worn? Why is personal protective equipment important?
Personal protective equipment Include: safety goggles face protection equipmet, gloves,
and lab coat/apron. For feet safety, shoe covers are advised During the carrying out of
any experiments Personal protective equipment should is worn so as to prevent the
spread of microbes and actual infections.
2. Why are there increasing levels of biological containment procedures? What level of biological
containment will be necessary to complete the experiments in this manual?
There are increasing levels of biological containment procedures for several reasons,
The main reason is to guarantee safety in the microbiology lab. There is also need to
prevent Laboratory Acquired Infections as well as the need to protect the scientists
working with pathogenic organisms and viruses in the course of their research. By
having biological containment preocedures in place, society is kept safe from
contamination too
3. What document should you refer to if you have questions regarding the safety or disposal of a
chemical? Where can this document be located?
In regarding the safety or disposal l the material safety data (MSDS) sheet is the point of
, lOM oA R c P S D| 30 87 312 9
Lab 1 Introduction to Science BIO250L
reference The MSDS can be found on the manufacturer’s website or in a binder placed
at a central place in our case the SDS can be found on
https://esciencelabs.com/educators/msds
4. List five safety procedures that should always be adhered to when working with microorganisms.
a. Hands must be washed thoroughly when one is done working with
microorganisms. b.
b. B. No Eating, drinking, smoking chewing of gum, smoking , touching contact
lenses, applying cosmetics, handling and storing food for human consumption
can be done in the laboratory
c. Before the start of an experiment all persons must read the instructions carefully.
d. Put on gloves before handling any specimen. Put on other PPE as needed,
people must wear closed shoes while in the lab
e. Decontaminate working surfaces after an experiment. Wash hands thoroughly
under running water
EXERCISE 1: DATA INTERPRETATION
1. What patterns do you observe based on the information in Table 4?
The more acidic the medium, the less microbes there are. As the PH approaches
neutral, the number of microbes increases and peaks at the ph of 7-Neutral. This trend
changes as soon as the ph starts to hit the alkalinic PH and number of microbes
continues to dwindle as the ph becomes more and more alkalinic.
2. Develop a hypothesis relating to the pH level of the culture media and the number of microbial
colonies observed in each culture.
Microbial activity increases and peaks as the ph approaches a ph of 7
3. What would your experimental approach be to test this hypothesis?
Set up a microbial culture and grow it under varying ph levels raning from most basic to
most acidic
4. What would be the independent and dependent variables?
The PH wold be the independent variable.
5. What would be your control?
A neutral ph would be the control
