BIOD 171/Microbiology Lab 3 Latest Exam Portage Learning Complete Guide Questions and Answers.

BIOD 171/Microbiology Lab 3
Latest Exam Portage Learning
Complete Guide Questions and
Answers.

, BIOD 171
L3: Lab Exam 3

Question 1
Not yet graded / 2.5 pts
List the four main steps used to prepare a WET mount, and indicate which (if any) step is
optional.
Your Answer:
1. Clean slide (70% ethanol)
2. Circle area on slide with wax hydrophobic pen
3. Apply organism to slide:

• If from culture, use sterile loop to spread onto slide.
• If from plate, use sterile loop to pick colonies and mix with a drop of distilled
water.

4. View under microscope.
1 – Clean slide
2 – Circle area on slide with wax/hydrophobic pen
3 – Apply organism to slide
4 – View under microscope
*Note: there are no optional steps in the wet mount.

Question 2
Not yet graded / 2.5 pts
Why is it important to first clean your slide before applying your sample?
Your Answer:
It is important to first clean your slide before applying the sample in order to remove
contaminants from the slide.
You must first remove any unwanted contaminants from the slide otherwise it may be
difficult to distinguish between the pathogen of interest and a contaminant.

Question 3
Not yet graded / 2.5 pts
When performing a wet mount technique, what is the advantage of using a wax or
hydrophobic pen?
Your Answer: