MIBO 3500 Final
For Escherichia coli, what is the genus and species name? -ANSW- genus:
Escherichia
species: Escherichia coli
What dutch merchant was the first to us a microscope to observe since-celled
microbes? What did he call these single-celled microbes? -ANSW- Leeuwenhoek,
Animalcules
The periplasmic space refers to what? -ANSW- Space between inner membrane and
outer membrane
True or False:
The key element in Pasteur's experiments disproving the theory of Spontaneous
Generation was his use of flasks having a swan neck that trapped contaminating
microbes in the air. -ANSW- True
True or False: Certain microbes in the soil accelerate the oxidation of metal sulfides in
groundwater from abandoned, subsurface mines, making the water extremely acidic. -
ANSW- True
The classification of all living things into three domains is based upon the analysis of: -
ANSW- 16S RNA sequence
True or False: As the wavelength of electromagnetic radiation increases, its penetrating
ability and resolving power increases. -ANSW- False
The staining technique to use on sputum from a suspected case of tuberculosis is: -
ANSW- Acid-fast stain
This term in microscopy is defined as the minimum distance between two objects where
they still appear separate. -ANSW- Resolution
Briefly explain how dark-field condensers creates a dark background for darkfield
microscopy. -ANSW- The objective picks up only scattered light from a specimen. If
light is un-scattered then no light enters the objective and the background will appear
dark due to the hollow cone of light created by dark-field condenser.
Differences in either _________ or ________ can result in a smooth or rough
appearance to bacterial colonies growing on an agar plate. -ANSW- 1. Length of O-
polysaccharide of LPS
2. Presence or absence of capsule (glycocalyx)
, Is the interior of a phospholipid bilayer hydrophobic or hydrophillic? -ANSW-
hydrophobic
What are the molecules that fill gaps between fatty acid side chains in bacterial cell
membranes? -ANSW- Hopanoids
Name two ways that bacteria and archaea cell membranes differ. -ANSW- 1. Ether
links instead of Ester links between glycerol and fatty acids.
2. Hydrocarbon chains are branched terpenoids.
By what mechanism does O2 and CO2 cross the cell membrane? -ANSW- diffusion
Would you expect Mycoplasma pneumoniae to be susceptible to penicillin? Explain. -
ANSW- No, it lacks a cell wall, and peptidoglycan is a target of penicillin.
Peptidoglycan consists of linear polymers of alternating __________ and _________
that are cross-linked by _________. -ANSW- N-acetylglucosamine
N-acetylmuramic acid
peptide chain
Your research collaborator is comparing environmental isolates from pond water
with those from tidal pools and estuaries along the Georgia coast. He reports
abundant growth of bacterial colonies from the pond water but sees no growth from
samples from tidal pools and estuaries when cultured on the same agar medium as
used for the pond water samples. You immediately spot a problem the experiment.
What do you tell the collaborator to do differently with the tidal pool and estuary
samples? Be specific. -ANSW- Try increasing the salt concentration in the medium to
match that in seawater
How would you classify the yeast strains we discussed in the case study with
respect to their requirement for molecular oxygen?
Would you predict that yeast have the enzymes catalase and superoxide
dismutase? -ANSW- Facultative anaerobes
What fundamental laboratory technique did Robert Koch develop that enabled him
to establish the causative agent of diseases such as anthrax and cholera? -ANSW-
Pure culture/Culture on solid media
True or False: Facilitated diffusion utilizes a membrane transport protein
to move solute molecules into the cell against the concentration gradient of the
solute. -ANSW- False
Active transport by an ABC transporter is powered by -ANSW- ATP
What are differential growth media? Give an example. -ANSW- Media that differentiate
characteristics of an organism. Blood agar (lysis),
For Escherichia coli, what is the genus and species name? -ANSW- genus:
Escherichia
species: Escherichia coli
What dutch merchant was the first to us a microscope to observe since-celled
microbes? What did he call these single-celled microbes? -ANSW- Leeuwenhoek,
Animalcules
The periplasmic space refers to what? -ANSW- Space between inner membrane and
outer membrane
True or False:
The key element in Pasteur's experiments disproving the theory of Spontaneous
Generation was his use of flasks having a swan neck that trapped contaminating
microbes in the air. -ANSW- True
True or False: Certain microbes in the soil accelerate the oxidation of metal sulfides in
groundwater from abandoned, subsurface mines, making the water extremely acidic. -
ANSW- True
The classification of all living things into three domains is based upon the analysis of: -
ANSW- 16S RNA sequence
True or False: As the wavelength of electromagnetic radiation increases, its penetrating
ability and resolving power increases. -ANSW- False
The staining technique to use on sputum from a suspected case of tuberculosis is: -
ANSW- Acid-fast stain
This term in microscopy is defined as the minimum distance between two objects where
they still appear separate. -ANSW- Resolution
Briefly explain how dark-field condensers creates a dark background for darkfield
microscopy. -ANSW- The objective picks up only scattered light from a specimen. If
light is un-scattered then no light enters the objective and the background will appear
dark due to the hollow cone of light created by dark-field condenser.
Differences in either _________ or ________ can result in a smooth or rough
appearance to bacterial colonies growing on an agar plate. -ANSW- 1. Length of O-
polysaccharide of LPS
2. Presence or absence of capsule (glycocalyx)
, Is the interior of a phospholipid bilayer hydrophobic or hydrophillic? -ANSW-
hydrophobic
What are the molecules that fill gaps between fatty acid side chains in bacterial cell
membranes? -ANSW- Hopanoids
Name two ways that bacteria and archaea cell membranes differ. -ANSW- 1. Ether
links instead of Ester links between glycerol and fatty acids.
2. Hydrocarbon chains are branched terpenoids.
By what mechanism does O2 and CO2 cross the cell membrane? -ANSW- diffusion
Would you expect Mycoplasma pneumoniae to be susceptible to penicillin? Explain. -
ANSW- No, it lacks a cell wall, and peptidoglycan is a target of penicillin.
Peptidoglycan consists of linear polymers of alternating __________ and _________
that are cross-linked by _________. -ANSW- N-acetylglucosamine
N-acetylmuramic acid
peptide chain
Your research collaborator is comparing environmental isolates from pond water
with those from tidal pools and estuaries along the Georgia coast. He reports
abundant growth of bacterial colonies from the pond water but sees no growth from
samples from tidal pools and estuaries when cultured on the same agar medium as
used for the pond water samples. You immediately spot a problem the experiment.
What do you tell the collaborator to do differently with the tidal pool and estuary
samples? Be specific. -ANSW- Try increasing the salt concentration in the medium to
match that in seawater
How would you classify the yeast strains we discussed in the case study with
respect to their requirement for molecular oxygen?
Would you predict that yeast have the enzymes catalase and superoxide
dismutase? -ANSW- Facultative anaerobes
What fundamental laboratory technique did Robert Koch develop that enabled him
to establish the causative agent of diseases such as anthrax and cholera? -ANSW-
Pure culture/Culture on solid media
True or False: Facilitated diffusion utilizes a membrane transport protein
to move solute molecules into the cell against the concentration gradient of the
solute. -ANSW- False
Active transport by an ABC transporter is powered by -ANSW- ATP
What are differential growth media? Give an example. -ANSW- Media that differentiate
characteristics of an organism. Blood agar (lysis),
