ASCP Exam Questions and Answers Actual Test 100%
Correct
Which is required to generate cDNA
A. DNA polymerase
B. RNase H
C. Restriction Enzyme
D. Helicase
B
What is the function of Taq Polymerase
recognize dsDNA
You have a 50 μL primer stock at 9pM/μl and want dilute the stock to 900nM. How
much stock should you add(what is the dilution factor)
2
What gene causes hypersensitivity to Irinotecan?
UGT1A
Which allele causes hypersensitivity to Carbamazepine?
HLA-b*1502
What gives MLPA its specificity
Stuffer Sequence
How is copy number determined?
MLPA
What is the gene that cause resistance to Vancomycin
vanA
Which assay is FISH best used for
Prader Willi
RNA polymerase II: where is it and what does it do
Eukaryotic: It catalyzes the transcription of DNA to synthesize precursors of mRNA and
most snRNA and microRNA. (transcribes mRNA)
Hazard that is blue and is level 4 what does it mean
big health hazard
Dr calls wanting test results but results have not been signed out by a medical
director what do you do
don't give out the result until it has been signed out my the MD
What trinucleotide repeat is involved in Fragile X?
CGG
In Fragile X, what is considered a full mutation expansion?
200+
What method is used to detect Fragile X?
Southern blot (now PCR /mPCR)
Which gene is the largest and has deletions/duplications as the most common
mutation types
DMD
Which HCV geneotype(s) has/have the best prognosis
Genotypes 2 &3
,Positive attributes of bDNA
Can be used for both DNA and RNA
What type of primer set is best?
forward primer should complement DNA strand and reverse primer should mirror and
inversely complement the forward primer
What are some things that stabilize RNA?
Inactivated and minimizing exposure to environmental/endogenous RNases
mRNA: 5'cap
What is the concentration of DNA with an OD of 2.0
100 μg/mL
Given the following data what can quantifiably be said about DNA when 260 = 1.4
and 280 = 1.1
There is 70ng of pure DNA
Sample is contaminated with proteins and phenols
What is the equation for PCR efficiency
(1+Σ)^n
You have a gel where the positive control worked, the negative control worked,
patient 1 had a double band, patient 2 was positive, and patient 3 had no bands.
What would you do?
repeat patient 3
How do you detect for methylation
Bisulfate sequencing
MSI (Micro-Satellite Instability) tests for what?
Mismatch Repair Genes
Translocation at t(15, 17)
Translation at t(15, 17)
Coumadin can be affect by a mutation in what gene
CYP2C19-KVORC1
What virus interfere with kidney transplant patients
BK
You have 2 HIV viral load tests 5 days apart 1 being high on day one and 1 being
low by day 5. What do you do?
Look into storage and transport
You are given a strand of primers. Calculate the Tm
2˚(AT) + 4˚(GC)
When doing a multiplex you observe faint bands. What do you do
Adjust the annealing temp
What do you do when you want to optimize a PCR reaction that generates short
PCR fragments?
Adjust annealing temperature
Which is caused by a T-cell rearrangement
- Mantle Cell
- Burkits
- Hodkins Lymphoma
- Sezary
, Sezary
What protects DNA as it is being replicated
Single Stranded Binding Protein
What opens the DNA double helix up
Helicase
What unwinds spiral DNA
topoisomerase
What does HCV assays target
5' UTR region
How does TaqMan work?
fluorescence is generated when the quencher is hydrolyzed
Which is the best order to limit contamination
- Mastermix/ patient/ positive/ negative
- Patient/ Mastermix/ negative/positive
- Matermix/ negative/ patient/ positive
- Mastermix/ positive/ patient/ negative
Matermix/ negative/ patient/ positive
How does Luminex work
Tests one patient with many beads
How do histones bind to DNA
ionic charges
Histones are associated with what amino acids
Lysine & Arginine
Methylation and Histone Modification are examples of what
epigenetics
Variations in Maternal & Paternal DNA mean what
imprinting
HLA typing over serology has the advantage of detecting what
null mutations
Doctor suspects that a patient has clonality you run a PCR and gel and the
patient sample, the positive control, and the negative control all show a band.
What do you do
repeat testing
How do you remove RNases from glassware
treat with DECP for 4 hours
Which strain of HPV is pathogenic
16, 18
What medication is used to tumor with a mutation in HER2/neu
Herceptin
What gene causes the majority of cancers
p53
What is the mutation for Fac V Leiden
1691G>A
What does Fac V Leiden cause
Deep Vein Thrombosis
Correct
Which is required to generate cDNA
A. DNA polymerase
B. RNase H
C. Restriction Enzyme
D. Helicase
B
What is the function of Taq Polymerase
recognize dsDNA
You have a 50 μL primer stock at 9pM/μl and want dilute the stock to 900nM. How
much stock should you add(what is the dilution factor)
2
What gene causes hypersensitivity to Irinotecan?
UGT1A
Which allele causes hypersensitivity to Carbamazepine?
HLA-b*1502
What gives MLPA its specificity
Stuffer Sequence
How is copy number determined?
MLPA
What is the gene that cause resistance to Vancomycin
vanA
Which assay is FISH best used for
Prader Willi
RNA polymerase II: where is it and what does it do
Eukaryotic: It catalyzes the transcription of DNA to synthesize precursors of mRNA and
most snRNA and microRNA. (transcribes mRNA)
Hazard that is blue and is level 4 what does it mean
big health hazard
Dr calls wanting test results but results have not been signed out by a medical
director what do you do
don't give out the result until it has been signed out my the MD
What trinucleotide repeat is involved in Fragile X?
CGG
In Fragile X, what is considered a full mutation expansion?
200+
What method is used to detect Fragile X?
Southern blot (now PCR /mPCR)
Which gene is the largest and has deletions/duplications as the most common
mutation types
DMD
Which HCV geneotype(s) has/have the best prognosis
Genotypes 2 &3
,Positive attributes of bDNA
Can be used for both DNA and RNA
What type of primer set is best?
forward primer should complement DNA strand and reverse primer should mirror and
inversely complement the forward primer
What are some things that stabilize RNA?
Inactivated and minimizing exposure to environmental/endogenous RNases
mRNA: 5'cap
What is the concentration of DNA with an OD of 2.0
100 μg/mL
Given the following data what can quantifiably be said about DNA when 260 = 1.4
and 280 = 1.1
There is 70ng of pure DNA
Sample is contaminated with proteins and phenols
What is the equation for PCR efficiency
(1+Σ)^n
You have a gel where the positive control worked, the negative control worked,
patient 1 had a double band, patient 2 was positive, and patient 3 had no bands.
What would you do?
repeat patient 3
How do you detect for methylation
Bisulfate sequencing
MSI (Micro-Satellite Instability) tests for what?
Mismatch Repair Genes
Translocation at t(15, 17)
Translation at t(15, 17)
Coumadin can be affect by a mutation in what gene
CYP2C19-KVORC1
What virus interfere with kidney transplant patients
BK
You have 2 HIV viral load tests 5 days apart 1 being high on day one and 1 being
low by day 5. What do you do?
Look into storage and transport
You are given a strand of primers. Calculate the Tm
2˚(AT) + 4˚(GC)
When doing a multiplex you observe faint bands. What do you do
Adjust the annealing temp
What do you do when you want to optimize a PCR reaction that generates short
PCR fragments?
Adjust annealing temperature
Which is caused by a T-cell rearrangement
- Mantle Cell
- Burkits
- Hodkins Lymphoma
- Sezary
, Sezary
What protects DNA as it is being replicated
Single Stranded Binding Protein
What opens the DNA double helix up
Helicase
What unwinds spiral DNA
topoisomerase
What does HCV assays target
5' UTR region
How does TaqMan work?
fluorescence is generated when the quencher is hydrolyzed
Which is the best order to limit contamination
- Mastermix/ patient/ positive/ negative
- Patient/ Mastermix/ negative/positive
- Matermix/ negative/ patient/ positive
- Mastermix/ positive/ patient/ negative
Matermix/ negative/ patient/ positive
How does Luminex work
Tests one patient with many beads
How do histones bind to DNA
ionic charges
Histones are associated with what amino acids
Lysine & Arginine
Methylation and Histone Modification are examples of what
epigenetics
Variations in Maternal & Paternal DNA mean what
imprinting
HLA typing over serology has the advantage of detecting what
null mutations
Doctor suspects that a patient has clonality you run a PCR and gel and the
patient sample, the positive control, and the negative control all show a band.
What do you do
repeat testing
How do you remove RNases from glassware
treat with DECP for 4 hours
Which strain of HPV is pathogenic
16, 18
What medication is used to tumor with a mutation in HER2/neu
Herceptin
What gene causes the majority of cancers
p53
What is the mutation for Fac V Leiden
1691G>A
What does Fac V Leiden cause
Deep Vein Thrombosis
