(MB) ASCP Practice Exam Questions Verified Answers
2023/2024
A cerebrospinal fluid (CSF) sample for hematologic evaluation should be tested within
how many hours of collection?
1 hour
2 hours
3 hours
4 hours Answ - 1 hour
A DNA specimen was sent to your Molecular Diagnostics Laboratory for DNA
sequencing, in order to determine whether patient A has a particular mutation in gene X.
The gene sequence is known. However, the gene mutation itself is not known.
Therefore, DNA sequencing cannot be performed on this DNA specimen. (True or
False)
True
False Answ - True
A parent has an autosomal dominant disorder. What percent chance does this parent
have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% Answ - 50%
A parent has an autosomal recessive disorder. What percent chance does this parent
have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% Answ - 100%
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods would best accomplish this
task?
Methylation-specific PCR
Standard PCR
PFGE
,RAPD PCR Answ - Standard PCR
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR indicates that patient has
Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene Answ - 85 CAG repeats in the Huntington gene
A reverse dot blot is best performed by:
-Immobilizing multiple unlabeled probes onto the membrane and then allowing one
labeled sample to hybridize to the unlabeled probes
-Immobilizing one labeled probe onto the membrane and allowing multiple labeled
samples to hybridize to the labeled probe
-Immobilizing multiple unlabeled probes onto the membrane and allowing multiple
unlabeled samples to hybridize to the unlabeled probes
-Allowing a single labeled sample to hybridize to multiple labeled single stranded probes
onto the membrane Answ - -Immobilizing multiple unlabeled probes onto the membrane
and then allowing one labeled sample to hybridize to the unlabeled probes
A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. If the absorbance at 280 nm gave a reading of 1.406, and if the the DNA
extract was re-suspended in 0.800 mL of EDTA solution, the DNA yield is: Answ -
(2.545 * 50ng/mcl)= 127.25
(127.25ng/mcl * 0.30) = 38.175ng/mcl
38.175ng/mcl ( 0.800mL1000) =
3054 micrograms
A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. The concentration of DNA in this extract is:
76.35 micrograms/mL
3817.5 micrograms/mL
381.75 micrograms/mL
3054.0 micrograms/mL Answ - 3817.5 micrograms/mL
According to Chargaff's rule of base pairing, adenine pairs with: Answ - Thymine
, According to the wobble hypothesis, a U in the 5' position of the anit-codon can pair
with:
U or C
A or G
A, C or G
Only A Answ - A or G
Acute promyelocytic leukemia (APL) is responsible for what translocation? Answ -
t(15;17)
After an amplification procedure followed by gel electrophoresis, you took a photo of
your gel. You notice consistent bands in all your gel lanes. However, you also see what
appears to be a faint but noticeable band in your reagent blank lane. What is your best
explanation for the reagent blank band?
-Taq polymerase concentration was too low in this PCR reaction
-There was obvious contamination in this PCR reaction
-The primers concentration was too high in this PCR reaction
-The dNTPs concentration was too high in this PCR reaction Answ - There was obvious
contamination in this PCR reaction
All of the following are components of nucleic acids, EXCEPT:
Phosphate group
Sugar (ribose or deoxyribose)
Nitrogenous base (A, G, C, T)
Formamide Answ - Formamide
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt's lymphoma
Acute promyelocytic leukemia Answ - Ewing sarcoma
All of the following are trinucleotide repeat disorders, EXCEPT:
Spinocerebellar Ataxia Type 8
Huntington Disease
Asperger Disease
Fragile X Disease Answ - Asperger Disease
All of the following enzymes are part of the DNA replication machinery, EXCEPT:
Ligase
DNA polymerase
Luciferase
Helicase Answ - Luciferase
2023/2024
A cerebrospinal fluid (CSF) sample for hematologic evaluation should be tested within
how many hours of collection?
1 hour
2 hours
3 hours
4 hours Answ - 1 hour
A DNA specimen was sent to your Molecular Diagnostics Laboratory for DNA
sequencing, in order to determine whether patient A has a particular mutation in gene X.
The gene sequence is known. However, the gene mutation itself is not known.
Therefore, DNA sequencing cannot be performed on this DNA specimen. (True or
False)
True
False Answ - True
A parent has an autosomal dominant disorder. What percent chance does this parent
have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% Answ - 50%
A parent has an autosomal recessive disorder. What percent chance does this parent
have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% Answ - 100%
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods would best accomplish this
task?
Methylation-specific PCR
Standard PCR
PFGE
,RAPD PCR Answ - Standard PCR
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR indicates that patient has
Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene Answ - 85 CAG repeats in the Huntington gene
A reverse dot blot is best performed by:
-Immobilizing multiple unlabeled probes onto the membrane and then allowing one
labeled sample to hybridize to the unlabeled probes
-Immobilizing one labeled probe onto the membrane and allowing multiple labeled
samples to hybridize to the labeled probe
-Immobilizing multiple unlabeled probes onto the membrane and allowing multiple
unlabeled samples to hybridize to the unlabeled probes
-Allowing a single labeled sample to hybridize to multiple labeled single stranded probes
onto the membrane Answ - -Immobilizing multiple unlabeled probes onto the membrane
and then allowing one labeled sample to hybridize to the unlabeled probes
A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. If the absorbance at 280 nm gave a reading of 1.406, and if the the DNA
extract was re-suspended in 0.800 mL of EDTA solution, the DNA yield is: Answ -
(2.545 * 50ng/mcl)= 127.25
(127.25ng/mcl * 0.30) = 38.175ng/mcl
38.175ng/mcl ( 0.800mL1000) =
3054 micrograms
A technologist uses the spectrophotometer to quantify the amount of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to
be 2.545. The concentration of DNA in this extract is:
76.35 micrograms/mL
3817.5 micrograms/mL
381.75 micrograms/mL
3054.0 micrograms/mL Answ - 3817.5 micrograms/mL
According to Chargaff's rule of base pairing, adenine pairs with: Answ - Thymine
, According to the wobble hypothesis, a U in the 5' position of the anit-codon can pair
with:
U or C
A or G
A, C or G
Only A Answ - A or G
Acute promyelocytic leukemia (APL) is responsible for what translocation? Answ -
t(15;17)
After an amplification procedure followed by gel electrophoresis, you took a photo of
your gel. You notice consistent bands in all your gel lanes. However, you also see what
appears to be a faint but noticeable band in your reagent blank lane. What is your best
explanation for the reagent blank band?
-Taq polymerase concentration was too low in this PCR reaction
-There was obvious contamination in this PCR reaction
-The primers concentration was too high in this PCR reaction
-The dNTPs concentration was too high in this PCR reaction Answ - There was obvious
contamination in this PCR reaction
All of the following are components of nucleic acids, EXCEPT:
Phosphate group
Sugar (ribose or deoxyribose)
Nitrogenous base (A, G, C, T)
Formamide Answ - Formamide
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt's lymphoma
Acute promyelocytic leukemia Answ - Ewing sarcoma
All of the following are trinucleotide repeat disorders, EXCEPT:
Spinocerebellar Ataxia Type 8
Huntington Disease
Asperger Disease
Fragile X Disease Answ - Asperger Disease
All of the following enzymes are part of the DNA replication machinery, EXCEPT:
Ligase
DNA polymerase
Luciferase
Helicase Answ - Luciferase
