MLT ASCP BOARD EXAM LATEST 2023-2024 ACTUAL EXAM 100 QUESTIONS AND
CORRECT ANSWERS WITH RATIONALES
Which of the following are not considered normal flora of the gastrointestinal tract:
A. Lactobacillus
B. Clostridium
C. Peptostreptococcus
D. Shigella - ANSWER-D;
Shigella and Salmonella are not found as normal flora in the gastrointestinal
tract.
True/False
The nuclear appendage at the tip of the arrow is a normal finding in females but
not in males. - ANSWER-True
The small drumstick-like nuclear extension (Barr body) marked by the blue arrow
is a chromatin mass which may represent a chromosomal remnant.
These nuclear appendages are found in normal XX females, but would not be
present in normal XY males. If such an appendage is found in a phenotypic male,
Klinefelter's syndrome (XXY male) may be identified clinically. The appendage
has a "drumstick" morphology.
Which nitrogen base would bind with a guanine nucleotide in forming double-
stranded DNA?
A. Cytosine
B. Uracil
C. Thymine
D. Adenine - ANSWER-A;
,MLT ASCP BOARD EXAM LATEST 2023-2024 ACTUAL EXAM 100 QUESTIONS AND
CORRECT ANSWERS WITH RATIONALES
In DNA complementary base pairing, guanine and cytosine pair and adenine and
thymine base pair. Remembering the phrase "G-CAT" helps one recollect correct
pairing.
A spinal fluid that is slightly hazy is briefly examined microscopically. The
technologist performing the count decides to make a 1:10 dilution using 30 µL of
sample. What volume of diluent should be used?
A. 70 µL
B. 270 µL
C. 300 µL - ANSWER-B;
To make a 1 : 10 dilution, 270 µL of diluent should be added to 30 µL of sample, .
Which of these blood levels will increase during intravascular hemolysis?
A. Serum (plasma) LDH
B. Serum (plasma) bilirubin
C. Reticulocytes
D. All of the above
E. None of the above - ANSWER-D;
Intravascular hemolysis is typically associated with increased levels of serum
(plasma) LDH and bilirubin, and an increased number of reticulocytes. Serum
LDH is found in higher levels during intravascular hemolysis due to fact that high
levels of LDH are normally found within the red cells, but is now being spilled into
the bloodstream via red cell lysis. Bilirubin is a breakdown product of hemoglobin,
which has also been spilled into the bloodstream from broken red blood cells via
hemolysis. Reticulocytosis is a reflection of the release of an increased number of
immature red blood cells from the marrow to account for the red blood cells that
are lost through hemolysis. This is a normal response. Failure to show an increased
,MLT ASCP BOARD EXAM LATEST 2023-2024 ACTUAL EXAM 100 QUESTIONS AND
CORRECT ANSWERS WITH RATIONALES
reticulocyte count with hemolytic episodes or hemorrhage would indicate an
ineffective erythropoiesis (possible bone marrow function problem).
True/False
Streptococcus pneumoniae is the most common cause of fatal community-acquired
pneumonia. - ANSWER-True
According to the paper by Jedrzejas, S. pneumoniae indeed is the cause of most
fatal cases of community-acquired pneumonia.
A slow, progressive drift of values away from the mean is called a:
A. Coefficient of Deviation
B. Dispersion
C. Trend
D. Variance
E. Shift - ANSWER-C;
A slow, progressive drift of values away from the mean is called a trend. Trends
occur when a particular analyte value slowly and progressively changes in one
direction from one run to the next. Monitoring data for trends is part of good
laboratory practice and is one indicator of many that helps laboratorians determine
whether the measurement system is fucntioning appropriately.
Which of the following would be the most appropriate method to confirm a
positive protein from a urine dipstick:
A. Immunoelectropheresis
B. Heat precipitation
C. Sulfosalicylic acid precipitation
, MLT ASCP BOARD EXAM LATEST 2023-2024 ACTUAL EXAM 100 QUESTIONS AND
CORRECT ANSWERS WITH RATIONALES
D. Protein electrophoresis - ANSWER-C;
Protein in urine can be confirmed using sulfosalicylic acid (SSA) precipitation.
The SSA reagent is added to a small volume of urine. Acidification causes
precipitation of protein in the sample, which is subjectively graded as trace, 1+, 2+,
3+ or 4+. SSA reaction will detect albumin, globulins, and Bence-Jones proteins.
Patients who develop severe sepsis or septic shock commonly have __________
plasma lactic acid values.
A. Decreased
B. Normal
C. Increased - ANSWER-C;
Patients who develop severe sepsis or septic shock commonly have increased
plasma lactic acid values.
When AHG or Coombs serum is used to demonstrate that red cells are antibody
coated in vivo, the procedure is termed:
A. Indirect technique
B. Direct technique
C. Hemagglutination technique
D. Hemolysis technique - ANSWER-B;
DAT ( Direct Antiglobulin Test ).
The assay which is most helpful in identifying specific allergens is:
A. Complement fixation
CORRECT ANSWERS WITH RATIONALES
Which of the following are not considered normal flora of the gastrointestinal tract:
A. Lactobacillus
B. Clostridium
C. Peptostreptococcus
D. Shigella - ANSWER-D;
Shigella and Salmonella are not found as normal flora in the gastrointestinal
tract.
True/False
The nuclear appendage at the tip of the arrow is a normal finding in females but
not in males. - ANSWER-True
The small drumstick-like nuclear extension (Barr body) marked by the blue arrow
is a chromatin mass which may represent a chromosomal remnant.
These nuclear appendages are found in normal XX females, but would not be
present in normal XY males. If such an appendage is found in a phenotypic male,
Klinefelter's syndrome (XXY male) may be identified clinically. The appendage
has a "drumstick" morphology.
Which nitrogen base would bind with a guanine nucleotide in forming double-
stranded DNA?
A. Cytosine
B. Uracil
C. Thymine
D. Adenine - ANSWER-A;
,MLT ASCP BOARD EXAM LATEST 2023-2024 ACTUAL EXAM 100 QUESTIONS AND
CORRECT ANSWERS WITH RATIONALES
In DNA complementary base pairing, guanine and cytosine pair and adenine and
thymine base pair. Remembering the phrase "G-CAT" helps one recollect correct
pairing.
A spinal fluid that is slightly hazy is briefly examined microscopically. The
technologist performing the count decides to make a 1:10 dilution using 30 µL of
sample. What volume of diluent should be used?
A. 70 µL
B. 270 µL
C. 300 µL - ANSWER-B;
To make a 1 : 10 dilution, 270 µL of diluent should be added to 30 µL of sample, .
Which of these blood levels will increase during intravascular hemolysis?
A. Serum (plasma) LDH
B. Serum (plasma) bilirubin
C. Reticulocytes
D. All of the above
E. None of the above - ANSWER-D;
Intravascular hemolysis is typically associated with increased levels of serum
(plasma) LDH and bilirubin, and an increased number of reticulocytes. Serum
LDH is found in higher levels during intravascular hemolysis due to fact that high
levels of LDH are normally found within the red cells, but is now being spilled into
the bloodstream via red cell lysis. Bilirubin is a breakdown product of hemoglobin,
which has also been spilled into the bloodstream from broken red blood cells via
hemolysis. Reticulocytosis is a reflection of the release of an increased number of
immature red blood cells from the marrow to account for the red blood cells that
are lost through hemolysis. This is a normal response. Failure to show an increased
,MLT ASCP BOARD EXAM LATEST 2023-2024 ACTUAL EXAM 100 QUESTIONS AND
CORRECT ANSWERS WITH RATIONALES
reticulocyte count with hemolytic episodes or hemorrhage would indicate an
ineffective erythropoiesis (possible bone marrow function problem).
True/False
Streptococcus pneumoniae is the most common cause of fatal community-acquired
pneumonia. - ANSWER-True
According to the paper by Jedrzejas, S. pneumoniae indeed is the cause of most
fatal cases of community-acquired pneumonia.
A slow, progressive drift of values away from the mean is called a:
A. Coefficient of Deviation
B. Dispersion
C. Trend
D. Variance
E. Shift - ANSWER-C;
A slow, progressive drift of values away from the mean is called a trend. Trends
occur when a particular analyte value slowly and progressively changes in one
direction from one run to the next. Monitoring data for trends is part of good
laboratory practice and is one indicator of many that helps laboratorians determine
whether the measurement system is fucntioning appropriately.
Which of the following would be the most appropriate method to confirm a
positive protein from a urine dipstick:
A. Immunoelectropheresis
B. Heat precipitation
C. Sulfosalicylic acid precipitation
, MLT ASCP BOARD EXAM LATEST 2023-2024 ACTUAL EXAM 100 QUESTIONS AND
CORRECT ANSWERS WITH RATIONALES
D. Protein electrophoresis - ANSWER-C;
Protein in urine can be confirmed using sulfosalicylic acid (SSA) precipitation.
The SSA reagent is added to a small volume of urine. Acidification causes
precipitation of protein in the sample, which is subjectively graded as trace, 1+, 2+,
3+ or 4+. SSA reaction will detect albumin, globulins, and Bence-Jones proteins.
Patients who develop severe sepsis or septic shock commonly have __________
plasma lactic acid values.
A. Decreased
B. Normal
C. Increased - ANSWER-C;
Patients who develop severe sepsis or septic shock commonly have increased
plasma lactic acid values.
When AHG or Coombs serum is used to demonstrate that red cells are antibody
coated in vivo, the procedure is termed:
A. Indirect technique
B. Direct technique
C. Hemagglutination technique
D. Hemolysis technique - ANSWER-B;
DAT ( Direct Antiglobulin Test ).
The assay which is most helpful in identifying specific allergens is:
A. Complement fixation
