Lab 8 Quiz – Microbiology
Question 1
The word ‘antigen’ is actually a combination for what two words?
Your Answer:
Antibody pathogen
Antibody-generating
Question 2
For the ELISA assay depicted below, which step (A-E) contains only specifically bound
antigens (aka a 'pure' population)?
Your Answer:
In step C you see only specifically bound antigens, aka a pure population. In step B you
see specific and non-specific antigens, but then the wash step removes the non-specific
or unbound antigens, leaving only the specific antigen which is present in step C.
Specifically bound antigens are present in step C. Specific and non-specific
antigens are present in B but then the wash step removes the non-specific
(unbound) antigens, leaving only the specific population seen in step C.
Question 3
What would happen in terms of the colorimetric readout if you forgot to do the wash step
after Step D (above)?
Your Answer:
If you forgot to do the was step after Step D, all the wells would have a dark color. This is
because the intensity of the readout is dependent upon the amount of the labeled
secondary antibody present in the well. Since the unbound secondary antibody was not
washed away, all the wells would react the same.
All of the wells would have a dark color. The intensity of the readout is
dependent upon the amount of the labeled secondary antibody present in the
well. Since the unbound secondary antibody was not washed away, all wells
would react equally.
Question 4
In a western blot ____________ are separated based on its size.
Your Answer:
Proteins
proteins
Question 5
When setting up a western blot, what is the purpose of the blocking step post-transfer?
This study source was downloaded by 100000801626315 from CourseHero.com on 09-07-2022 23:22:50 GMT -05:00
https://www.coursehero.com/file/60489555/lab-8-lab-exam-docx/
Question 1
The word ‘antigen’ is actually a combination for what two words?
Your Answer:
Antibody pathogen
Antibody-generating
Question 2
For the ELISA assay depicted below, which step (A-E) contains only specifically bound
antigens (aka a 'pure' population)?
Your Answer:
In step C you see only specifically bound antigens, aka a pure population. In step B you
see specific and non-specific antigens, but then the wash step removes the non-specific
or unbound antigens, leaving only the specific antigen which is present in step C.
Specifically bound antigens are present in step C. Specific and non-specific
antigens are present in B but then the wash step removes the non-specific
(unbound) antigens, leaving only the specific population seen in step C.
Question 3
What would happen in terms of the colorimetric readout if you forgot to do the wash step
after Step D (above)?
Your Answer:
If you forgot to do the was step after Step D, all the wells would have a dark color. This is
because the intensity of the readout is dependent upon the amount of the labeled
secondary antibody present in the well. Since the unbound secondary antibody was not
washed away, all the wells would react the same.
All of the wells would have a dark color. The intensity of the readout is
dependent upon the amount of the labeled secondary antibody present in the
well. Since the unbound secondary antibody was not washed away, all wells
would react equally.
Question 4
In a western blot ____________ are separated based on its size.
Your Answer:
Proteins
proteins
Question 5
When setting up a western blot, what is the purpose of the blocking step post-transfer?
This study source was downloaded by 100000801626315 from CourseHero.com on 09-07-2022 23:22:50 GMT -05:00
https://www.coursehero.com/file/60489555/lab-8-lab-exam-docx/
