Attempt History
Attempt Time Score
LATEST Attempt 1 42 minutes 22.25 out of 30
Score for this quiz: 22.25 out of 30
Submitted Jun 2 at 8:44pm
This attempt took 42 minutes.
Question 1
pts
The word ‘antigen’ is actually a combination for what two words?
Your Answer:
combination of antibody and gen means which produces . so it means when antigen
enters the body produces of antibodies will start
Antibody-generating
Question 2
pts
For the ELISA assay depicted below, which step (A-E) first contains only specifically
bound antigens (aka a 'pure' population)?
Your Answer:
C
Specifically bound antigens are present in step C. Specific and non-specific
antigens are present in B but then the wash step removes the non-specific
(unbound) antigens, leaving only the specific population seen in step C.
Question 3
pts
What would happen in terms of the colorimetric readout if you forgot to do the wash step
after Step D (above)?
This study source was downloaded by 100000801626315 from CourseHero.com on 09-07-2022 23:24:20 GMT -05:00
https://www.coursehero.com/file/96114161/BIOD-171-Lab8-examdocx/
, Your Answer:
the unbound secondary antibody will not be washed away, so we may have false
positive result
All of the wells would have a dark color. The intensity of the readout is dependent
upon the amount of the labeled secondary antibody present in the well. Since the
unbound secondary antibody was not washed away, all wells would react equally.
Question 4
2..5 pts
A western blot separates proteins based on ______________.
Your Answer:
Size and molecular mass
Size
Question 5
2..5 pts
When setting up a western blot, what is the purpose of the blocking step post-transfer?
Your Answer:
it can prevent antibodies from binding to membrane non specifically
Blocking prevents non-specific binding of the antibody—you only want the
antibody to bind to its specific target.
Question 6
1..5 pts
When developing a western blot what two factors influence the intensity of the band?
Your Answer:
type of membrane , intensity of current supplied
Both the amount of protein loaded as well as the duration (time) of development
is directly proportional to band intensity.
Question 7
.5 pts
Identify (specific name) given to the region of the western blot indicated by the arrow.
This study source was downloaded by 100000801626315 from CourseHero.com on 09-07-2022 23:24:20 GMT -05:00
https://www.coursehero.com/file/96114161/BIOD-171-Lab8-examdocx/
Attempt Time Score
LATEST Attempt 1 42 minutes 22.25 out of 30
Score for this quiz: 22.25 out of 30
Submitted Jun 2 at 8:44pm
This attempt took 42 minutes.
Question 1
pts
The word ‘antigen’ is actually a combination for what two words?
Your Answer:
combination of antibody and gen means which produces . so it means when antigen
enters the body produces of antibodies will start
Antibody-generating
Question 2
pts
For the ELISA assay depicted below, which step (A-E) first contains only specifically
bound antigens (aka a 'pure' population)?
Your Answer:
C
Specifically bound antigens are present in step C. Specific and non-specific
antigens are present in B but then the wash step removes the non-specific
(unbound) antigens, leaving only the specific population seen in step C.
Question 3
pts
What would happen in terms of the colorimetric readout if you forgot to do the wash step
after Step D (above)?
This study source was downloaded by 100000801626315 from CourseHero.com on 09-07-2022 23:24:20 GMT -05:00
https://www.coursehero.com/file/96114161/BIOD-171-Lab8-examdocx/
, Your Answer:
the unbound secondary antibody will not be washed away, so we may have false
positive result
All of the wells would have a dark color. The intensity of the readout is dependent
upon the amount of the labeled secondary antibody present in the well. Since the
unbound secondary antibody was not washed away, all wells would react equally.
Question 4
2..5 pts
A western blot separates proteins based on ______________.
Your Answer:
Size and molecular mass
Size
Question 5
2..5 pts
When setting up a western blot, what is the purpose of the blocking step post-transfer?
Your Answer:
it can prevent antibodies from binding to membrane non specifically
Blocking prevents non-specific binding of the antibody—you only want the
antibody to bind to its specific target.
Question 6
1..5 pts
When developing a western blot what two factors influence the intensity of the band?
Your Answer:
type of membrane , intensity of current supplied
Both the amount of protein loaded as well as the duration (time) of development
is directly proportional to band intensity.
Question 7
.5 pts
Identify (specific name) given to the region of the western blot indicated by the arrow.
This study source was downloaded by 100000801626315 from CourseHero.com on 09-07-2022 23:24:20 GMT -05:00
https://www.coursehero.com/file/96114161/BIOD-171-Lab8-examdocx/
