LE7 College aantekeningen Kidney Physiology (NWI-BM079) - Animal Models

LE 7 – Animal Models of Kidney
Disease
December 6 t h , 2023

Why do we use animal models




Disease models
Drosophila
 Low costs
 Generation time: 10 days
 Invertebrate
 Malpighian tubules
 Fast knock down

Malphigian tubules

Osmoregulatory system of insects. Has tubules that branch from the gut. Absorption and secretion of
ions, minerals, and water. Developmental processes are partially conserved.

, Zebrafish
 Low costs
 Generation time: 3 months
 Vertebrate
 Kidney: 2 nephrons
 Fast gene knock down

Nephron segmentati on
Zebrafish have 2 nephrons. Nephron
segmentation is largely conserved. Protein
expression is similar in the conserved
segments. Zebrafish don’t need hoop of Henle
and collecting duct  Freshwater fish don’t
need to conserve fluid. They want to get rid of
as much fluid as possible.




Example: expression of NCC in the DCT.