BMSC 240 Sample final
1. "What is a definition of ""salting out"
Making proteins less soluble by increasing the salt concentration
2. Which type of chromatography was described in lecture to allow DNA separation from
RNA?
Ion-exchange
3. What protein loading dye component adds density?
Glycerol
4. What is not a type of human antibody?
IgT
5. What part of an antigen is bound by an antibody?
Epitope
6. Which plasmid type would allow survival in the presence of ampicillin?
Resistance
7. "When doing a restriction digest, always add the enzyme ______."
Last
8. True or False: A lower percentage agarose gel is suited for separation of larger DNA
fragments.
True
9. PulseNet tracks foodborne illnesses using what method?
Pulsed-field Gel Electrophoresis
10. What is not used to precipitate DNA?
Phenol
11. Which PCR temperature is variable by individual experiment?
Annealing
12. The main reason a non-Taq polymerase might be used in PCR is:
It makes less mistakes when copying
13. What blood type will not show agglutination in standard blood testing?
AB
14. Which method uses vibration to lyse cells?
Sonication
15. A restriction enzyme
1. "What is a definition of ""salting out"
Making proteins less soluble by increasing the salt concentration
2. Which type of chromatography was described in lecture to allow DNA separation from
RNA?
Ion-exchange
3. What protein loading dye component adds density?
Glycerol
4. What is not a type of human antibody?
IgT
5. What part of an antigen is bound by an antibody?
Epitope
6. Which plasmid type would allow survival in the presence of ampicillin?
Resistance
7. "When doing a restriction digest, always add the enzyme ______."
Last
8. True or False: A lower percentage agarose gel is suited for separation of larger DNA
fragments.
True
9. PulseNet tracks foodborne illnesses using what method?
Pulsed-field Gel Electrophoresis
10. What is not used to precipitate DNA?
Phenol
11. Which PCR temperature is variable by individual experiment?
Annealing
12. The main reason a non-Taq polymerase might be used in PCR is:
It makes less mistakes when copying
13. What blood type will not show agglutination in standard blood testing?
AB
14. Which method uses vibration to lyse cells?
Sonication
15. A restriction enzyme
