BIOL202 Week 3 Lab 3 Culturing and Aseptic Technique APU

Lab3:CulturingandAsepticTechnique(100points)  Eachquestiononthelabworksheetmustbeansweredcompletely,thoroughly,incompletesentencesand correctlyin orderto beconsideredforfullcredit  Ifthequestionasksyoutodoresearchorfindasource,areputable,credibleand/orscholarlysourcecitationmustbeincluded inorder tobeconsidered for fullcredit  Ifamathformulaisrequiredtoarrivetoananswer,workmustbeshownotherwise,nocreditwill beawarded Pre-LabQuestions 1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What isthe experimental way you can test your practices to confirm that you are using properaseptictechnique? (5 points) 2. Inalaboratorysetting,whatarethreewaysyoucanproperlysterilizeculturingequipment?(5points) 3. Foreachinoculationtool,giveonescenarioinwhichuseofthattoolwouldbeappropriate. (5points) Experiment1ResultsTables Experiment1Post-LabQuestions 1. Do some research and try to identify one to three types of microbes cultured on yourplates. Using any resources available to you (i.e., a textbook or internet sources such asthe CDC website), look up what bacteria are usually found on the areas from which youobtained your samples and the morphological characteristics of their colonies. Describewhat morphological traits (i.e., size, shape, arrangement, color, margin, etc.) led to yourhypothesized species identification. Be as specific as possible in the microbe type. (5points) 2. ForthecolonieshypothesizedinQuestion1,specifywhichplateandsourcethemicrobes came from. Are you surprised to find this type of microbe on this surface? (5points) 3. Looking at your negative control, did you perform proper aseptic technique or were yourplates contaminated? If contamination did occur, list the possible sources and how youcanpreventcontaminationin the future.(5 points) 4. Was there a large risk for airborne contamination of your experimental plates (look atyour airborne control plate)? Based on the colonies that grew on your plates, do youthinkanyofyour experimentplatesreceivedairbornecontamination?(5points) Experiment2ResultsTables  Table2:InitialReservedPlateColonyGrowthObservations(15points)  Table3:FinalPlateandStabTubeGrowthObservations(15points) Experiment2Post-LabQuestions 1. Were all of your colony transfers successful? Explain what could have been the cause ofanyunsuccessfultransfers.(5points) 2. Did you have any growth that was different in appearance from the initial plates? Whatmightaccountforanydifferencesingrowthonthetransferplate/tube?(5points) 3. Describe why an unsuccessful transfer or growth of the transfer plate/tube differing fromthe initial plates would be a problem if this experiment were placed in a larger context(i.e., only onestep in alongerexperiment). (5points) 4. Do some research and describe two or three scenarios in which it would be preferable touse a stab tube vs. a growth plate. (Hint: What do bacteria use to help them move? Canmotility be used to help identify many medically important pathogenic bacteria such astheEnterobacteriaceae?)(5 points)