BIOL202 Week 5 Lab 5 Growth of Microorganisms APU

Lab5:GrowthofMicroorganisms(100points)  Eachquestiononthelabworksheetmustbeansweredcompletely,thoroughly,incompletesentencesand correctlyin orderto beconsideredforfullcredit  Ifthequestionasksyoutodoresearchorfindasource,areputable,credibleand/orscholarlysourcecitationmustbeincluded inorder tobeconsidered for fullcredit  Ifamathformulaisrequiredtoarrivetoananswer,workmustbeshownotherwise,nocreditwill beawarded Pre-LabQuestions 1. Why don’t microorganisms in cultures exhibit constant exponential growth? What aresome steps you could take to extend the lifespan of a microbial culture? (5 points) 2. Using a textbook or a reputable online source, describe how lab cultures are maintainedin a continual pattern of growth. Focus particularly on those used in biotechnology, suchas E. coli, which is used to make human insulin. (5 points). 3. What are the four phases of bacterial growth in a closed system?(5 points) 4. What are the physical and chemical methods of microbial control and what are the twomethods used?(5 points). 5. You are a physician trying to isolate bacterial colonies from the human gut in attempt todiagnoseagastrointestinalinfection.Youstreakyoursampleonagrowthmediacontaining glucose, amino acids, and salts that contain both sulfur and phosphorous witha pH of 7. You incubate the plates in aerobic conditions at 37 ˚C for three days, at whichpointyoucan seeclearbacterialcoloniesformingontheplate. .Experiment1ResultsTablesTable1:Experiment1Results(10points) Experiment1Post-LabQuestions 1. What does the pink band in the FTM indicate? (5 points). 2. What would be the effect of using FTM tubes with a broad pink zone? (5 points). 3. Provide an explanation as to why bacteria from each location displayed their pattern ofgrowth. Are there any results that surprise you? If so, why? (5 points). 4. Why is it important to pull the inoculating needle out of the FTM via the same path youinserted it? (5 points). 5. Why was it necessary to have separate controls for the agar plates and the FTM tubes?(5 points). 6. Experiment 2 Results Tables  Table2:Experiment2Results(10points) Experiment2Post-LabQuestions 1. Which germicide was most effective for each sample? Was the same germicide the mosteffective for microorganisms isolated from the different sources? 2. Which germicide was least effective? Was the same germicide the least effective formicroorganisms isolated from the different sources? What could explain this similarity ordifference? (5 points). 3. Were any of the cultured microorganisms particularly resistant to the germicides tested?If so, which culture? What could explain this strong resistance? (5 points). 4. What was the purpose of the dipped piece of filter paper in deionized water plate? (5points). 5. Researchthechemicalformulasforbleach,isopropylalcohol,andchlorhexidinegluconate (the active ingredient in Hibiclens®). How does each chemical achieve itsgermicidal properties on the molecular level? (5 points). 6. What are some real-world applications for a study like this? For instance, how wouldthese results influence cleaning practices in a doctor’s office or treatment of a disease?(5 points).