BIO 250L Lab 2 Culturing & Aseptic Technique Straighterline FALL 2023

Pre-lab Questions 1. This lab includes two experiments that each look at different aspects of culturing microorganisms onto growth media. What concepts does each experiment focus on? Why do you think these concepts are important to the field of microbiology? Click here to enter text. 2. In this lab, the experiments will allow or call for the use of four inoculation tools. List these tools and provide a scenario in which you would use each tool. ○ Click here to enter text. ○ Click here to enter text. ○ Click here to enter text.. ○ Click here to enter text. 3. Describe a piece of equipment used in biotech labs to extend the growth pattern of microbes. Focus particularly on those used with E. coli used to make human insulin. Click here to enter text. 4. You’re a physician trying to isolate bacterial colonies from the human gut in an attempt to diagnose a gastrointestinal infection. You streak your sample on a growth media containing glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7. You incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see clear bacterial colonies forming on the plate. Would you feel confident in stating that you had successfully cultured all the bacteria from your gut sample? Why or why not? Click here to enter text. EXPERIMENT 1: AGAR PLATE PREPARATION AND BACTERIAL INOCULATION Introduction Questions 1. Describe the objectives of Experiment 1. State the goal of the experiment and the learning outcome that it is trying to convey to you as a microbiology student. “Lab 2 Culturing & Aseptic Technique BIO250L” Click here to enter text. 2. Proper aseptic technique is crucial to ensuring the growth of pure bacterial colonies. What will you do in this lab to demonstrate that you are using proper aseptic technique? Click here to enter text. In a laboratory setting, what are three ways you can properly sterilize culturing equipment? ○ Click here to enter text. ○ Click here to enter text. ○ Click here to enter text. 3. What method of sterilization will you use in this experiment?