CITI TRAINING
Biosafety Level 3
Practices & Procedures
COMPLETED EXAM w/
RATIONALES
2024
,1. What is the main difference between BSL-2 and BSL-3
in terms of laboratory design and practices?
a) BSL-3 requires negative air pressure and HEPA
filtration of exhaust air.
b) BSL-3 requires biosafety cabinets and personal
protective equipment for all procedures.
c) BSL-3 requires a separate changing room and shower
facility for personnel.
d) BSL-3 requires a double-door entry and an anteroom for
decontamination.
*Answer: a) BSL-3 requires negative air pressure and
HEPA filtration of exhaust air. Rationale: This is the main
feature that distinguishes BSL-3 from BSL-2, as it prevents
the escape of potentially infectious aerosols from the
laboratory.*
2. Which of the following agents is not typically handled in
a BSL-3 laboratory?
a) Mycobacterium tuberculosis
b) Bacillus anthracis
c) Ebola virus
d) Francisella tularensis
*Answer: c) Ebola virus. Rationale: Ebola virus is
classified as a Risk Group 4 agent, which requires BSL-4
, containment and practices.*
3. What is the most common route of exposure to
infectious agents in a BSL-3 laboratory?
a) Ingestion
b) Injection
c) Inhalation
d) Skin contact
*Answer: c) Inhalation. Rationale: Inhalation of aerosols or
droplets is the most likely and most hazardous route of
exposure in a BSL-3 laboratory, as many agents can cause
severe respiratory infections.*
4. What is the purpose of using a biosafety cabinet (BSC)
in a BSL-3 laboratory?
a) To protect the operator from exposure to infectious
agents.
b) To protect the environment from contamination by
infectious agents.
c) To protect the product or experiment from contamination
by other agents.
d) All of the above.
*Answer: d) All of the above. Rationale: A BSC provides a
physical barrier between the operator and the work
material, as well as an airflow system that prevents cross-
contamination and release of infectious agents.*
5. What type of personal protective equipment (PPE) is
required for working in a BSL-3 laboratory?
a) Gloves, gown, mask, and eye protection.
Biosafety Level 3
Practices & Procedures
COMPLETED EXAM w/
RATIONALES
2024
,1. What is the main difference between BSL-2 and BSL-3
in terms of laboratory design and practices?
a) BSL-3 requires negative air pressure and HEPA
filtration of exhaust air.
b) BSL-3 requires biosafety cabinets and personal
protective equipment for all procedures.
c) BSL-3 requires a separate changing room and shower
facility for personnel.
d) BSL-3 requires a double-door entry and an anteroom for
decontamination.
*Answer: a) BSL-3 requires negative air pressure and
HEPA filtration of exhaust air. Rationale: This is the main
feature that distinguishes BSL-3 from BSL-2, as it prevents
the escape of potentially infectious aerosols from the
laboratory.*
2. Which of the following agents is not typically handled in
a BSL-3 laboratory?
a) Mycobacterium tuberculosis
b) Bacillus anthracis
c) Ebola virus
d) Francisella tularensis
*Answer: c) Ebola virus. Rationale: Ebola virus is
classified as a Risk Group 4 agent, which requires BSL-4
, containment and practices.*
3. What is the most common route of exposure to
infectious agents in a BSL-3 laboratory?
a) Ingestion
b) Injection
c) Inhalation
d) Skin contact
*Answer: c) Inhalation. Rationale: Inhalation of aerosols or
droplets is the most likely and most hazardous route of
exposure in a BSL-3 laboratory, as many agents can cause
severe respiratory infections.*
4. What is the purpose of using a biosafety cabinet (BSC)
in a BSL-3 laboratory?
a) To protect the operator from exposure to infectious
agents.
b) To protect the environment from contamination by
infectious agents.
c) To protect the product or experiment from contamination
by other agents.
d) All of the above.
*Answer: d) All of the above. Rationale: A BSC provides a
physical barrier between the operator and the work
material, as well as an airflow system that prevents cross-
contamination and release of infectious agents.*
5. What type of personal protective equipment (PPE) is
required for working in a BSL-3 laboratory?
a) Gloves, gown, mask, and eye protection.
