Proteomics
N o t es
S t u d y
BSPO-364 Proteomics
,Handouts:
Proteomics.
BSPO-364.
References:
Principles of proteomics:
(By Richard M. Twyman).
Google search & Wikipedia.
BSPO-364 Proteomics
,Proteomics
PROTEIN:
Protein is a group of complex organic macromolecules that contain carbon, hydrogen, oxygen, nitrogen,
and usually sulfur and it is composed of one or more chains of amino acids. Proteins are fundamental
components of all living cells and include many substances, such as enzymes, hormones, and antibodies,
which are necessary for the proper functioning of an organism.
Amino acids
. Proteins are made out of 20 amino acids
The subunits of a protein are amino acids or to be precise amino acid
residues. An amino acid consists of a central carbon atom (the alpha Carbon
Calpha) and an amino group (NH2), a hydrogen atom (H), a carboxy group
(COOH) and a side chain (R) which are bound to the Calpha. Different side
chains (Ri) make up different amino acids with different chemical properties.
Peptide bond
A peptide bond is formed via covalent binding of the Carbon atom of the
Carboxy group of one amino acid to the nitrogen atom of the amino group of
another amino acid by condensation and in this process water molecule is
removed & its not “dehydration” which is the removal of pure water molecule
without any covalent bonding.
BSPO-364 Proteomics
, Structure of protein
Proteins can be found in four different structures or forms
Primary
Secondary
Tertiary
Quaternary
� Primary structure: the linear arrangment of amino acids in a protein
and the location of covalent linkages such as disulfide bonds between
amino acids.
� Secondary structure: areas of folding or coiling within a protein;
examples include alpha helices and b-pleated sheets, which are
stabilized by hydrogen bonding.
BSPO-364 Proteomics
N o t es
S t u d y
BSPO-364 Proteomics
,Handouts:
Proteomics.
BSPO-364.
References:
Principles of proteomics:
(By Richard M. Twyman).
Google search & Wikipedia.
BSPO-364 Proteomics
,Proteomics
PROTEIN:
Protein is a group of complex organic macromolecules that contain carbon, hydrogen, oxygen, nitrogen,
and usually sulfur and it is composed of one or more chains of amino acids. Proteins are fundamental
components of all living cells and include many substances, such as enzymes, hormones, and antibodies,
which are necessary for the proper functioning of an organism.
Amino acids
. Proteins are made out of 20 amino acids
The subunits of a protein are amino acids or to be precise amino acid
residues. An amino acid consists of a central carbon atom (the alpha Carbon
Calpha) and an amino group (NH2), a hydrogen atom (H), a carboxy group
(COOH) and a side chain (R) which are bound to the Calpha. Different side
chains (Ri) make up different amino acids with different chemical properties.
Peptide bond
A peptide bond is formed via covalent binding of the Carbon atom of the
Carboxy group of one amino acid to the nitrogen atom of the amino group of
another amino acid by condensation and in this process water molecule is
removed & its not “dehydration” which is the removal of pure water molecule
without any covalent bonding.
BSPO-364 Proteomics
, Structure of protein
Proteins can be found in four different structures or forms
Primary
Secondary
Tertiary
Quaternary
� Primary structure: the linear arrangment of amino acids in a protein
and the location of covalent linkages such as disulfide bonds between
amino acids.
� Secondary structure: areas of folding or coiling within a protein;
examples include alpha helices and b-pleated sheets, which are
stabilized by hydrogen bonding.
BSPO-364 Proteomics
