Paper chromatography
1
, PAPER CHROMATOGRAPHY
• Paper Chromatography (PC) was first introduced by
German scientist Christian Friedrich Schonbein
(1865).
• PC is considered to be the simplest and most widely
used of the chromatographic techniques because of
its applicability to isolation, identification and
quantitative determination of organic and inorganic
compounds.
2
, PAPER CHROMATOGRAPHY
• ANALYSIS OF UNKNOWN SUSTANCES
It is carried out mainly by the flow of solvents
on specially designed filter paper.
There are two types of paper chromatography,
they are:
3
, PAPER CHROMATOGRAPHY
1.PAPER ADSORPTION CHROMATOGRAPHY
Paper impregnated with silica or alumina acts as
adsorbent (stationary phase) and solvent as
mobile phase.
2.PAPER PARTITION CHROMATOGRAPHY
Moisture / Water present in the pores of
cellulose fibers present in filter paper acts as
stationary phase & another mobile phase is used
as solvent
In general P.C – Paper Partition
Chromatography
4
1
, PAPER CHROMATOGRAPHY
• Paper Chromatography (PC) was first introduced by
German scientist Christian Friedrich Schonbein
(1865).
• PC is considered to be the simplest and most widely
used of the chromatographic techniques because of
its applicability to isolation, identification and
quantitative determination of organic and inorganic
compounds.
2
, PAPER CHROMATOGRAPHY
• ANALYSIS OF UNKNOWN SUSTANCES
It is carried out mainly by the flow of solvents
on specially designed filter paper.
There are two types of paper chromatography,
they are:
3
, PAPER CHROMATOGRAPHY
1.PAPER ADSORPTION CHROMATOGRAPHY
Paper impregnated with silica or alumina acts as
adsorbent (stationary phase) and solvent as
mobile phase.
2.PAPER PARTITION CHROMATOGRAPHY
Moisture / Water present in the pores of
cellulose fibers present in filter paper acts as
stationary phase & another mobile phase is used
as solvent
In general P.C – Paper Partition
Chromatography
4
