ASCP EXAM LATEST 2024 QUESTIONS AND CORRECT
VERIFIED ANSWERS|GRADED A+
What types of containers are needed to collect PB, BM, and
ST/CV samples - ANSWER-pb: NH tube
bm: NH tube or syringe
ST/ CV: sterile container with antibiotics
How much PB is needed for a successful study?
(differs with age) - ANSWER-5-10mL for adults
3-5mL for infants
How much bm is needed for successful study? - ANSWER-1-
3mL
How much amniotic fluid is needed for successful study? -
ANSWER-15-30mL in 2 separate containers?
How much ST is needed for successful study? - ANSWER-•
Tissue:
CVS: 5-25 mg
,Skin: 1mm3 minimum
Tumor: 1mm3 minimum
What transportation temperature are lethal to cells -
ANSWER-Over 40 C
If transporting specimen over long term what temperature
should they be kept at - ANSWER-4C
How long can be PB samples be stored without being cultures
- ANSWER-1 week after collection at 4C
If a bone marrow cannot be set within 4 hours how should it
be stored? - ANSWER-add media and kept at room temp
Tissues should not be transported dry. What are the
preferred medias to keep them moist in order from best to
worst. - ANSWER-RPMI
PBS or Hanks(HBSS)
NaCL
How long can you store tissues? - ANSWER-Overnight at 4C.
Never fixed
,How should amniotic fluid be transported? - ANSWER-non-
toxic plastic container/syringes and deliver ASAP
How should CVS be transported - ANSWER-sterile media with
an antibiotics and FBS
How do you avoid cellular contamination in prenatal tissues?
- ANSWER-discard the fist 1-2 mL of amniotic fluid
How do you avoid cellular contamination in ST? - ANSWER-
dissect parenchyma and fatty tissue
Which specimen is the most widely used for CG analysis -
ANSWER-PB
True or false: Too much NH can inhibit cell growth - ANSWER-
true
What is the preferred anticoagulant? - ANSWER-NH
What anticoagulants are supposedly toxic to lymphocytes -
ANSWER-EDTA, LiH, ACD
, What chemicals substances prevent fungal infection -
ANSWER-Amphotericin B, Fungizone,
Mycostatin
What chemicals prevent bacterial infection - ANSWER-
penicillin/streptomycin
Kanamycin/gentamycin if resistant to above
What causes clotting in PB/BM samples (2) - ANSWER-Not
enough heparin: Make sure 100 units er 5mL of sample were
added
patients with a coagulation issue( thrombocytosis and
thrombocythemia and APL)
What causes cells density to be too high - ANSWER-
hyperplastic BM. Do dilution
What causes cell density to be too low - ANSWER-hypoplastic
Bm. Buffy coat
What is the goal time period to set a bone marrow sample -
ANSWER-4 hrs after collection
VERIFIED ANSWERS|GRADED A+
What types of containers are needed to collect PB, BM, and
ST/CV samples - ANSWER-pb: NH tube
bm: NH tube or syringe
ST/ CV: sterile container with antibiotics
How much PB is needed for a successful study?
(differs with age) - ANSWER-5-10mL for adults
3-5mL for infants
How much bm is needed for successful study? - ANSWER-1-
3mL
How much amniotic fluid is needed for successful study? -
ANSWER-15-30mL in 2 separate containers?
How much ST is needed for successful study? - ANSWER-•
Tissue:
CVS: 5-25 mg
,Skin: 1mm3 minimum
Tumor: 1mm3 minimum
What transportation temperature are lethal to cells -
ANSWER-Over 40 C
If transporting specimen over long term what temperature
should they be kept at - ANSWER-4C
How long can be PB samples be stored without being cultures
- ANSWER-1 week after collection at 4C
If a bone marrow cannot be set within 4 hours how should it
be stored? - ANSWER-add media and kept at room temp
Tissues should not be transported dry. What are the
preferred medias to keep them moist in order from best to
worst. - ANSWER-RPMI
PBS or Hanks(HBSS)
NaCL
How long can you store tissues? - ANSWER-Overnight at 4C.
Never fixed
,How should amniotic fluid be transported? - ANSWER-non-
toxic plastic container/syringes and deliver ASAP
How should CVS be transported - ANSWER-sterile media with
an antibiotics and FBS
How do you avoid cellular contamination in prenatal tissues?
- ANSWER-discard the fist 1-2 mL of amniotic fluid
How do you avoid cellular contamination in ST? - ANSWER-
dissect parenchyma and fatty tissue
Which specimen is the most widely used for CG analysis -
ANSWER-PB
True or false: Too much NH can inhibit cell growth - ANSWER-
true
What is the preferred anticoagulant? - ANSWER-NH
What anticoagulants are supposedly toxic to lymphocytes -
ANSWER-EDTA, LiH, ACD
, What chemicals substances prevent fungal infection -
ANSWER-Amphotericin B, Fungizone,
Mycostatin
What chemicals prevent bacterial infection - ANSWER-
penicillin/streptomycin
Kanamycin/gentamycin if resistant to above
What causes clotting in PB/BM samples (2) - ANSWER-Not
enough heparin: Make sure 100 units er 5mL of sample were
added
patients with a coagulation issue( thrombocytosis and
thrombocythemia and APL)
What causes cells density to be too high - ANSWER-
hyperplastic BM. Do dilution
What causes cell density to be too low - ANSWER-hypoplastic
Bm. Buffy coat
What is the goal time period to set a bone marrow sample -
ANSWER-4 hrs after collection
