2024 LATEST BIOD 171 Lab 9 - Notebook Unknown pathogen analysis

AH09 Unknown Pathogen Analysis Objective: To identify an unknown pathogen(s) based on the given assays developed during this course. Notes: • Two samples from the hospital was brought to your lab for diagnosis. After culturing the samples on LB agar plates another co- worker began the analysis. However, due to an emergency your co- worker has left the remaining work to you. Before leaving your co- worker informs you the 2 liquid culture samples were accidentally combined before the Gram stain was started. However, the 2 cultures remain separated on LB plates for future tests. o GOAL: To correctly identify the 2 pathogens. Results: • Gram (+) coccus clusters • Gram (-) bacillus rods • MacConkey plate o Culture A – no growth o Culture B – lactose fermentation is present ▪ Zone 1 – growth is apparent ▪ Zone 2- colony isolates begin to form. ▪ Zone 3 – single colony isolates. • BAP- o Culture A- beta-hemolysis o Culture B- no beta-hemolysis • EMB- o Culture A- No growth o Culture B- colonies with green metallic sheen • Catalase Test- o Culture A- positive o Culture B- positive