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Microbiology BOC test with correct answers

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a sheep blood and phenylethyl alcohol agars b eosin-methylene blue and sheep blood agars c thioglycollate broth and chocolate agar d chocolate and modified Thayer-Martin agars - ANSWER-d chocolate and modified Thayer-Martin agars d Chocolate agar and chocolate agar- based selective media should be used for recovery of Neisseria gonorrhoeae from urethral discharge. Chocolate agar provides the nutrients required by N gonorrhoeae and selective media contains antimicrobial agents that inhibits other organisms and permits recovery of pathogenic Neisseria. A sheep blood agar plate inoculated with 0.001mL of urine grows 70 colonies of Staphylococcus aureus. How many colony forming units per mL of urine be reported? a 70 b 700 c 7,000 d 70,000 - ANSWER-d 70,000 d When 0.001 mL of urine is plated, the growth of one colony is equivalent to 1000 CFU/mL. Thus 70 colonies is 70,000 CFU/mL. The lowest concentration of antibiotic that inhibits growth of a test that inhibits growth of a test organism is the: a minimum inhibitory concentration b serum inhibitory concentration c minimum bactericidal titer d maximum inhibitory titer - ANSWER-a minimum inhibitory concentration a The MIC is a basic laboratory measurement of the activity of an antibiotic against an organism. It is the

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