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Class notes Gpat

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Lecture notes of 31 pages for the course Gpat at Gpat (Pharmacology)

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B’ PHARMACY
SUBJECT: Pharmaceutical Biotechnology
Subject Code: BP605T
MODULE- 2



GENE CLONING VECTORS

PRESENTED BY Chamanpreet Kaur
Assistant Professor
Pharmaceutics
ASBASJSMCOP , BELA

, Objective of course
Genetic Engineering applications relation to production of pharmaceutical




• Learning Outcome

• Students will learn about cloning vectors. They will also learn
about the application of genetic Enginnering and various steps
of Polymerase Chain Reaction.

, INTRODUCTION

• A cloning vector is a DNA molecule in which
foreign DNA can be inserted or integrated and
which is further capable of replicating within
host cell to produce multiple clones of
recombinant DNA.

• Examples: Plasmids,phage or virus

, Characteristics

It should be able to replicate autonomously.
Origin of replication.
Selectable markers.
Restriction sites.

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February 27, 2024
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31
Written in
2023/2024
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Chamanpreet kaur
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