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Module 3 BIOD 171 Already Passed

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Module 3 BIOD 171 Already Passed True or False: A nanometeris longer than a micrometer. What is the total magnification (relative to your eye) of a sample imaged with a 60x objective and a 10x eyepiece? Show your math. Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance the magnification of a sample. A. Objective B. Condenser C. Iris diaphragm D. Eye piece What is the total magnification (relative to your eye) of a sample imaged with a 20x objective and a 15x eyepiece? Show your math. True or False: Staining is often required to image a cell that is adherent and flat (thin). Assuming a constant (non-adjustable) light source power, identify the part of the microscope you would adjust to limit the amount of light entering the microscope.Select all that apply. A. Objective B. Condenser C. Iris diaphragm D. Eye piece This type of microscope is best suited for visualizing GFP, RFP and YFP proteins. True or False: A cell that is adherent, flat (thin) and unstained is easily identified using bright field microscopy. True or False: The distinguishing characteristic of Gram-Negative bacteria is the presence of LPS in the outer membrane. True or False: If you wish to study the motility of an organism you cannot heat fix, but you can chemically fix the specimen. True or False: If a patient is suspected of having malaria, a Giemsa stain would be an appropriate differential test to perform. True or False: Growth media is best suited for distinguishing between two similar species of bacteria. A researcher is asked to determine which of two vials contains E coli and which contains Salmonella. Knowing both are Gram-Negative while only one of them is capable of fermenting lactose, which type of media would be best suited: A. Growth media B. Differential media C. Selective media D. Selective and Differential media True or False: LB agar is classified as a selective, non-differential media. True or False: A Giemsa stain can be used to determine the presence of pathogenic bacteria. . True or False: Following the decolorization step of the Gram stain, Gram-Negative bacteria will appear colorless.

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Module 3 BIOD 171 Already Passed

1). True or false: a nanometeris longer than a micrometer.

 Ans: False. A nanometer is 1,000 times smaller than a micrometer.


2). What is the total magnification (relative to your eye) of a sample imaged with a 60x
objective and a 10x eyepiece? show your math.

 Ans: 60 x 10 = 600x magnification


3). Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance
the magnification of a sample.
a. objective
b. condenser
c. iris diaphragm
d. eye piece

 Ans: A. Only the oculars (eyepiece) and the objectives contribute to the magnification
of the sample. Since the eyepiece is fixed, only the objectives could be altered.


4). What is the total magnification (relative to your eye) of a sample imaged with a 20x
objective and a 15x eyepiece? show your math.

 Ans: 20 x 15 = 300x magnification


5). True or false: staining is often required to image a cell that is adherent and flat (thin).

 Ans: True. Adherent, flat and unstained cells are almost invisible due to the limits on
both resolution and contrast. Therefore, cell staining is often required to adequately
image the sample.


6). Assuming a constant (non-adjustable) light source power, identify the part of the
microscope you would adjust to limit the amount of light entering the microscope.select all
that apply.

a. objective
b. condenser




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