BIO 250L Final Exam Questions And Answers 2024

What are some things to remember for lab safety? - correct answer--Wash hands and clean bench with Lysol before and after lab -No eating or drinking -Close toed shoes and pants, tie hair back, wear gloves -Never take cultures out of lab -Store bookbag at front of classroom -Don't put things down sink or in trash can, go in biohazard container -Don't open plates with fungal growth -Handle microscopes gently and clean before putting back Aseptic technique - correct answer-a set of procedures and carefully controlled conditions to prevent contamination by pathogens Characteristics of aseptic technique (7) - correct answer-1. Fire as a sterilizing tool 2. Use of adductive vertical air currents 3. Limit exposure of nutrient media 4. Keep silent and attentive while inoculating media 5. Avoid the creation of bacteria-containing aerosols 6. Storing and incubating solid medium plates inverted 7. Disinfecting work surfaces Pure culture - correct answer-a culture containing a single kind of microorganism Agar - correct answer-a gel-like polysaccharide compound used for culturing microbes; extracted from certain red algae Major microbiological culture media - correct answer-nutrient broths and agar plates Autotroph - correct answer-an organism that is able to form nutritional organic substances from simple inorganic substances such as carbon dioxide Heterotroph - correct answer-an organism that obtains carbon via organic carbon (previously fixed from CO2) in their environment Mixotroph - correct answer-capable of both autotrophy and heterotrophy; will engage in either of them depending on environmental conditions How to prepare a bacterial smear - correct answer-1. Pass both sides of the slide briefly through Bunsen burner flame 2. Transfer 5 loopfuls of bacteria onto slide 3. Allow smear to dry completely