(MB) ASCP PRACTICE EXAM QUSTIONS WITH
100% CORRECT ANSWERS VERIFIED (2024)
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answers ✅Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: -
correct answers ✅Thymine
What genes would be screened in a breast cancer panel? - correct
answers ✅HER2, ERBB2, BRCA1
Next Generation Sequencing uses: - correct answers ✅Short
sequence reads
,Purines and pyrimidines differ from each other in that: - correct
answers ✅Purines have two rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answers ✅Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answers ✅1 in
1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during
electrophoresis does not depend on which of the following factors?
Net charge of the molecule
,Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answers ✅Nucleotide
sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answers
✅Initiation, exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answers ✅3'-GAC
, A patient with impaired judgment, personality changes, signs of
abnormal body movements and depression comes to the physician's
office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood
specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR
indicates that patient has Huntington Disease, HD. Which of the
following would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - correct answers ✅85 CAG
repeats in the Huntington gene
Which two HPV types are responsible for most cases of cervical
cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - correct answers ✅16 and 18
100% CORRECT ANSWERS VERIFIED (2024)
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answers ✅Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: -
correct answers ✅Thymine
What genes would be screened in a breast cancer panel? - correct
answers ✅HER2, ERBB2, BRCA1
Next Generation Sequencing uses: - correct answers ✅Short
sequence reads
,Purines and pyrimidines differ from each other in that: - correct
answers ✅Purines have two rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answers ✅Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answers ✅1 in
1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during
electrophoresis does not depend on which of the following factors?
Net charge of the molecule
,Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answers ✅Nucleotide
sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answers
✅Initiation, exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answers ✅3'-GAC
, A patient with impaired judgment, personality changes, signs of
abnormal body movements and depression comes to the physician's
office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood
specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR
indicates that patient has Huntington Disease, HD. Which of the
following would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - correct answers ✅85 CAG
repeats in the Huntington gene
Which two HPV types are responsible for most cases of cervical
cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - correct answers ✅16 and 18
