Medical Laboratory Science …MEd, MLS (ASCP) UPDATED & FINAL Microbiology, Clinical Laboratory Science Review TEST (Micro), Clinical Microbiology Review TEST WITH COMPLETE SOLUTIONS

Cause Toxic Shock syndrome and food poisoning - ANSWER S. aureus (cutaneous infections) S. aureus is resistant to what antibiotic? - ANSWER Penicillin Common cause of hospital acquired UTI - ANSWER Coagulase-negative staphylococci (CNS) What is the hemolysis of S. aureus - ANSWER Beta hemolytic Novobiocin susceptible - ANSWER S. epidermidis Coagulase negative, Novobiocin Resistant Staphy - ANSWER S. Saprophyticus How does the catalase test work - ANSWER Converts hydrogen peroxide to oxygen and water Describe the pathogenicity of Group A Strep - ANSWER Group A Strep (Strep Pyogenes) 1. Strep throat 2. Rheumatic fever 3. Glomerulonephritis Oval GPC in chain and clusters - ANSWER Streptococcus spp Hemolysis of Group A Strep - ANSWER Usually Beta heolytic ( wide zone) What lysis RBCs, Platelets, and Neutrophiles in Group A Strep - ANSWER Streptolysin O and Streptolysin S To detect species that produce streptolysin O only, what must be done? - ANSWER Stab into agar and place a coverslip over inoculum or incubate anaerobically Group A is sensitive to what antibiotic? - ANSWER Bacitracin Group A is resistant and positive to? - ANSWER Resistant to SXT and Positive to PYR Most common cause of neonatal septicemia and meningitis - ANSWER Group B Strep ( S. agalactiae) Group B Strep: Resistant to? Positive? - ANSWER Resistant to Bacitraacin/SXT Positive for: Sodium Hippurate/CAMP S. bovis is part of what Lancefield Group - ANSWER Group D Streptococci Group D Strep nonenterococci is normal ----and --- flora - ANSWER fecal oral Lancefield group D - ANSWER Enterococcus Enterococcus are 80%-----E. --- 15%----E.---- - ANSWER 80% E. faecalis 15% E. faecium Enterococcus Hydrolyze Esculin---- Growth in 6.5% NaCl--- PYR---- - ANSWER Hydrolyze Esculin----Positive Growth in 6.5% NaCl---Yes PYR----Positive What are the pathogenicity of S. pneumoniae - ANSWER 1. Labor pneumonia 2. Otitis Media 3. Meningitis Gram Stain: Football Shaped - ANSWER S. pneumoniae What is the hemolysis of S. pneumoniae - ANSWER alpha-hemolytic S. pneumoniae Key Characteristics: 1. Bile Solubility 2. Optochin - ANSWER 1.Bile Solubility---Positive 2. Optochin:-----Sensitive What causes subacute bacterial endocarditis - ANSWER Viridan streptococci Viridan streptococci Key Characteristics: 1. Bile Solubility-- 2. Optochin--- - ANSWER Key Characteristics: 1. Bile Solubility-Neg 2. Optochin-Resistant Lysostaphin is used to differentiate Staphy from what other genus - ANSWER Micrococcus Fermentation of glucose (OF tube ) helps differentiate Staphy from? - ANSWER Micrococcus What other Staphy species in addition to S. aureus produces a coagulase positive result - ANSWER S. intermedius Slime production is associated with what Staphy species - ANSWER S. epidermidis What enzymes contribute to the virulence of Staphy? - ANSWER Hyaluronidase: damages basement membrane of tissues Beta-lactamase: produces strains that are able to inactivate penicillin and ampicillin Acetoin production helps distinguish what two staphy strains - ANSWER 1. S. aureus (+) 2. S. intermedius (-) Rhodotorula growth on SBA - ANSWER Pink-orange/red pigment Causative agent of pediatric tinea capitis but not in adults - ANSWER Microsporum audouinii Causes spontaneous abortions and meningitis in animals - ANSWER Listeria Monocytogenes What is the hemolysis of Listeria M - ANSWER Beta Hemolytic Translucent, gray How does Listeria Monocytogenes look on CAMP - ANSWER CAMP positive -Shovel (not arrowhead) Key Characteristics of L. monocytogenes 1. Catalase--- 2. Esculin---- 3. CAMP---