BIOD 171 Portage Microbiology Module 4 exam SPRING 2024

1. growth media nutrient-rich environment for isolating and culturing microorganisms - liquid or solid 2. LB media (lysogeny broth) Most common nutrient Broth Known for ability to grow vast array of microbes 3. Selective Media allows for growth of specific microbes, while restricting the growth of other microbes often used to grow human pathogens, or slower growing microbes. The selectivity allows for discounting foreign unwanted microbes 4. Differential Media allows growth of several types of microbes and displays visible differences among those microbes ex. both gram(-) ecoli v salmonella as ecoli ferments lactose and growth w lactose shows red, where salmonella remains white/tan 5. Enriched Media used to grow fastidious orgs - complex growth requirements that will cease to grow if not exact. 6. Agar Polysaccharide derived from seaweed extract Solid & smooth allowing growth of colonies in isolated dots Covering entire plate = lawn more agar = more firm/ 7. LB agar Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria; LBagar capable of wide variety of micro'o's. LB is non-selective and non-differential. 8. Trypticase Soy Agar TSA or TSAYE - identical in color to LBagar Multipurpose media w high variety Non-selective & non-differential 9. Blood Agar BAPlates, derivative of TSA combined with mammalian blood, at [5-10%] BAP are red