MCB2289l Week 11 Final Exam 2018

Question QUESTION 1 The process of forming mRNA from the template strand of DNA is called _____________ Transcription Replication Duplication Translation QUESTION 2 Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA ATTGCTGGA TTACCGCCA TAACGACCT TAACGTCCA QUESTION 3 Which test uses a medium that is both selective and differential for members of the genus Staphylococcus? MacConkey agar Mannitol Salt agar Coagulase test Bile esculin hydrolysis QUESTION 4 Which of the following processes removes or destroys endospores? Antisepsis Disinfection Sterilization Sanitization QUESTION 5 The purpose of heat fixation is to _______. Adjust the morphology of the organisms on the slide Encourage growth of bacterial cells on the slide Inactivate and adhere microorganisms placed on the slide Harden the bacteria so that they are not destroyed by the staining procedure QUESTION 6 Eukaryotes have membrane-bound organelles True False QUESTION 7 A pure culture contains a known number of organisms two types of known microorganisms one type of microorganism QUESTION 8 The internationally recognized reference for bacterial classification and identification is called Bergey's Manual True False QUESTION 9 How is a glass culture tube handled during aseptic transfers? The mouth is held in the flame while the inoculating tool is inserted for removal of organisms The cap is removed and placed on the table away from the flame The bottom of the tube is held in the flame for 10 seconds The mouth is passed through the flame right when opened and just before closing QUESTION 10 How is a respirometer used to measure fermentation in the Cellular Respiration experiment? By the amount of glucose consumed By the size of the gas bubble produced By the amount of ATP produced By the amount of gas consumed QUESTION 11 Which organelle is involved in the packaging of proteins for secretion? Rough endoplasmic reticulum Nucleus Golgi apparatus Mitochondrion QUESTION 12 Which statement correctly describes the level of contamination on different objects? Different objects will increase in their level of contamination with described temperature Different objects will have the same level of contamination. Environments in contact with animals and humans are likely to be less contaminated due to hygiene practices. Different objects will vary in their level of contamination. QUESTION 13 Which type of reaction(s) requires oxygen as a substrate? Anaerobic Aerobic Both aerobic and anaerobic reactions require oxygen Neither reactions require oxygen QUESTION 14 Catalase breaks hydrogen peroxide down in water and carbon dioxide True False QUESTION 15 Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar. They are glucose fermenters They are lactose fermenters They produce urease They reduce nitrate to nitrite QUESTION 16 Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT Nitrate reduction Acid production Gas production Fermentation QUESTION 17 All of the following are common to all cells at some point in their development EXCEPT: DNA The cell wall The cell membrane Ribosomes QUESTION 18 In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound in referred to as the _______________? mordant decolorizer counterstain primary stain QUESTION 19 All of the following are true of an enzyme-catalyzed reaction EXCEPT? An enzyme reduces the activation energy Every time a substrate is converted to a product, a bit of the enzyme is used up The enzyme speeds up the conversion of substrate to product Enzymes are not used up in the chemical reaction QUESTION 20 Which of the following diseases are caused by acid-fast bacterium: HIV Influenza Cholera Tuberculosis (TB) QUESTION 21 If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test will be red, indicating a positive result. True False QUESTION 22 Immersion oil must only be used with which objective lens? 4x 10x 40x 100x QUESTION 23 An antimicrobial, such as isoniazid, that is only effective against mycobacterial species would be referred to as a __________ drug. Broad spectrum Narrow spectrum Intermediate spectrum Naturally occurring QUESTION 24 Most human pathogens are classified as thermophiles. True False QUESTION 25 In living organisms, information for making proteins flows from _______? RNA to DNA to protein. RNA to protein to DNA DNA to RNA to protein Protein to RNA to DNA QUESTION 26 Microorganisms can be described as being ubiquitous which means that they: All grow uniformly on an agar plate Are present on nearly every surface Can only grow on solid surfaces Can only grow in the absence of oxygen QUESTION 27 Stains are commonly used for all of the following EXCEPT The pathogen identification process The diagnosis of disease The classification of organisms The treatment of disease QUESTION 28 The monomers that make up DNA molecules are called _______? Sugars Amino acids Nucleotides Nitrogenous bases QUESTION 29 Which of the following is a chemical means of controlling microbial growth? Disinfectant High temperature Scrubbing action Ultraviolet radiation QUESTION 30 All of the following organisms are expected to grow within an anaerobe jar EXCEPT Obligate anaerobe Aerotolerant anaerobe Obligate aerobe Facultative anaerobe QUESTION 31 The cloudy growth seen in a broth culture is referred to as Precipitate Pellicle Sediment Turbidity QUESTION 32 An antibiotic which interferes with the activity of ribosomes would have the most effect on which of the following? DNA replication The cell wall Protein synthesis Cell membrane function QUESTION 33 When a large zone of no growth is found around an antibiotic disc being used for identification purposed, this generally means that the microbe is ______ to the antibiotic Resistant Pathogenic Sensitive Neutral QUESTION 34 All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT? Streak plating Loop dilution pour plating Gram staining Serial dilution followed by spread plating QUESTION 35 The smallest living units of the human body are __________. molecules atoms tissues cells QUESTION 36 When first viewing a specimen using the brightfield microscope, it is recommended that you begin viewing the specimen with which objective lens? 100X 40X 10X 4X (scanning objective) QUESTION 37 Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture. True False QUESTION 38 In addition to linking together nucleotides to synthesize a daughter strand, DNA polymerase also ________ Unzips the parent DNA molecule "proofreads" the order of nucleotides Makes the new DNA strand form a helix "zips" the parent molecule back together QUESTION 39 All of the following are found in most prokaryotic cells EXCEPT Cell membrane Cytoplasm Cell wall Nucleus QUESTION 40 The sources of all antimicrobial drugs are fungi and other microorganisms. True False QUESTION 41 Which carbohydrate is the primary or preferred starting compound for glycolysis and the fermentation pathway? Sucrose Glucose Lactose fructose QUESTION 42 All of the following body sites normally have large numbers of resident microbiota EXCEPT? Nose Large intestine Skin Blood QUESTION 43 Human skin provides an environment with NaCl levels slightly above that of most bacterial cells. Which organism would most likely be found on the skin? A halotolerant organism An organism that does not tolerate hypertonic salt conditions very well An obligate anaerobe A psychrophile QUESTION 44 Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink True False QUESTION 45 An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____ characteristics that can be helpful in the identification of the microorganism. Morphological Genotypic Physiological Immunological QUESTION 46 A hypertonic solution is prepared by the addition of sucrose. Which of the following would happen to bacteria placed in this solution: The bacterial cells will swell, but not lyse There will be no change in the bacterial cells The bacterial cells will undergo osmotic lysis The bacterial cells will undergo plasmolysis QUESTION 47 Which of the following items would be disposed of into the sharps container? Used paper towels Used gloves Bacterial culture plates Used microscope slides QUESTION 48 When is it crucial to wear protective eyewear when working in the lab? When using the microscope When using a Bunsen burner When working with hazardous chemicals When working with sharp objects QUESTION 49 The term aerobic, anaerobic, and microaerophilic are used to categorize microorganisms based on their ______ requirements. oxygen temperature carbon dioxide pH QUESTION 50 The ability to distinguish fine detail or discriminate between two very closely spaced objects is called: Microscopy Resolution Clarification magnification