BIOL 395 Final Exam Questions with Correct Answers

What is the macromolecule concentration that is being measured in the Bradford Assay lab (lab 1)? - ANSWER-Protein concentration is measure in the lab. The name of the protein is BSA or Bovine Serum Albumin. diluent - ANSWER-solution - usually buffer or water - with which the sample is diluted. aliquot - ANSWER-A portion of a well-mixed sample removed for testing (a sub amount of the total. dilution factor - ANSWER-how diluted a solution is compared with the starting solution of same composition (DF=final volume/aliquot volume used) concentration - ANSWER-amount of solute/volume; generally solute is solid before going into solution, therefore usually reported using units such as g/l; mg/ml or M. stock solution - ANSWER-the solution is kept at a high concentration and made in large quantities, ready to be used for multiple protocols after correct dilutions. working solution - ANSWER-the solution that is at the concentration needed for the experiment. serial dilution - ANSWER-starting from stock solution, making a series of dilutions, using aliquots from each new dilution to make the next dilution. Usually done in regular steps, i.e. making a series of dilutions from 1: 10- 1:10,000 to determine optimal enzyme concentration. final volume - ANSWER-the total volume of the solution. FV = aliquot + diluent molarity - ANSWER-the number of moles / liter solution What type of pipette is used to measure 7 ml? - ANSWER-A serological pipette is used. To separate components of a reaction, you need to ________________________. - ANSWER-centrifuge To mix component of a reaction, you need to_______________________. - ANSWER-vortex When letting a reaction sit for a specific amount of time it is called ____________________________. - ANSWER-incubate