WJEC & Eduqas Notes
Enzymes
, WJEC Unit 1
Eduqas Core Concepts
STUDY CHECKLIST AND ASSESSMENT
OBJECTIVES
Instructions
Regular revision throughout the year is essential. It’s vital you keep a track of what you
understand and what you don’t understand. This booklet is designed to help you do this.
Use the following key to note how well you understand the work after your revision. Put the
letter R, A or G in the table. If you place an R or an A then you should make a note of what
you are struggling with and the end of this book under the relevant section and seek help
with this.
Key
R = Red. I am not confident about my knowledge and understanding
A = Amber. I am fairly confident about my knowledge and understanding
G = green. I am very confident about my knowledge and understanding
, Enzymes
1. Can you describe the structure of an enzyme.
2. Can you describe the lock and key hypothesis and the induce fit model of enzyme action.
3. Can you accurately describe the shape of a substrate compared to the shape of the active site.
4. Can you describe and give examples of an anabolic and a catabolic reaction.
5. Do you understand the term: Activation Energy.
6. Can you explain how enzymes lower the activation energy of a reaction.
7. Can you draw an energy graph to show the progress of a chemical reaction with and without an enzym
8. Can you state the factors that affect the rate of an enzyme catalysed reaction.
9. Can you draw a graph to show the effects of these factors from point 8 on the rate of an enzyme
catalysed reaction.
10. Can you explain these graphs from point 9.
11. Can you distinguish between the structure and the mode of action of competitive and
non-competitive inhibitors.
12. Do you know which inhibitor is affected by an increase in substrate concentration.
13. Can you explain how an increase in substrate concentration can affect the inhibitor in point 12.
, 14. Can you calculate the rate of an enzyme catalysed reaction from a graph.
Medical And Industrial Applications Of Enzymes
15. Can you define what an immobilised enzyme is?
16. Can you describe several ways in which enzymes are immobilised?
17. Can you distinguish between a qualitative and a quantitative biosensor?
18. Can you describe the functions of the various components of a quantitative (digital) biosensor?
19. Can you list the advantages of a digital biosensor?
20. Can you describe a medical use of a biosensor?
21. Can you describe the industrial production of lactose free milk using immobilised enzymes?
22. Can you explain the effects of bead size and flow rate on how much product is formed during the
industrial use of immobilised enzymes.
23. Can you list the advantages of using immobilised enzymes in industrial processes?
24. Can you explain the effect of increasing temperature on immobilised enzymes and compare this to
non immobilised (free) enzymes.
25. Can you apply your understanding of enzymes to unfamiliar scenarios?
3
Assessment Objective Description
AO1 Demonstrate knowledge and understanding of scientific ideas, processes, te
AO2 Apply knowledge and understanding of scientific ideas, processes, techniqu
• In a theoretical context
• In a practical context
• When handling qualitative data
• When handling quantitative data
AO3 Analyse, interpret and evaluate scientific information, ideas and evidence, i
to: • Make judgments and reach conclusions
• Develop and refine practical design and procedures
4
WORD BANK
Below is a list of some key words and phrases you will need to learn and understand in this immobilised
Enzymes
, WJEC Unit 1
Eduqas Core Concepts
STUDY CHECKLIST AND ASSESSMENT
OBJECTIVES
Instructions
Regular revision throughout the year is essential. It’s vital you keep a track of what you
understand and what you don’t understand. This booklet is designed to help you do this.
Use the following key to note how well you understand the work after your revision. Put the
letter R, A or G in the table. If you place an R or an A then you should make a note of what
you are struggling with and the end of this book under the relevant section and seek help
with this.
Key
R = Red. I am not confident about my knowledge and understanding
A = Amber. I am fairly confident about my knowledge and understanding
G = green. I am very confident about my knowledge and understanding
, Enzymes
1. Can you describe the structure of an enzyme.
2. Can you describe the lock and key hypothesis and the induce fit model of enzyme action.
3. Can you accurately describe the shape of a substrate compared to the shape of the active site.
4. Can you describe and give examples of an anabolic and a catabolic reaction.
5. Do you understand the term: Activation Energy.
6. Can you explain how enzymes lower the activation energy of a reaction.
7. Can you draw an energy graph to show the progress of a chemical reaction with and without an enzym
8. Can you state the factors that affect the rate of an enzyme catalysed reaction.
9. Can you draw a graph to show the effects of these factors from point 8 on the rate of an enzyme
catalysed reaction.
10. Can you explain these graphs from point 9.
11. Can you distinguish between the structure and the mode of action of competitive and
non-competitive inhibitors.
12. Do you know which inhibitor is affected by an increase in substrate concentration.
13. Can you explain how an increase in substrate concentration can affect the inhibitor in point 12.
, 14. Can you calculate the rate of an enzyme catalysed reaction from a graph.
Medical And Industrial Applications Of Enzymes
15. Can you define what an immobilised enzyme is?
16. Can you describe several ways in which enzymes are immobilised?
17. Can you distinguish between a qualitative and a quantitative biosensor?
18. Can you describe the functions of the various components of a quantitative (digital) biosensor?
19. Can you list the advantages of a digital biosensor?
20. Can you describe a medical use of a biosensor?
21. Can you describe the industrial production of lactose free milk using immobilised enzymes?
22. Can you explain the effects of bead size and flow rate on how much product is formed during the
industrial use of immobilised enzymes.
23. Can you list the advantages of using immobilised enzymes in industrial processes?
24. Can you explain the effect of increasing temperature on immobilised enzymes and compare this to
non immobilised (free) enzymes.
25. Can you apply your understanding of enzymes to unfamiliar scenarios?
3
Assessment Objective Description
AO1 Demonstrate knowledge and understanding of scientific ideas, processes, te
AO2 Apply knowledge and understanding of scientific ideas, processes, techniqu
• In a theoretical context
• In a practical context
• When handling qualitative data
• When handling quantitative data
AO3 Analyse, interpret and evaluate scientific information, ideas and evidence, i
to: • Make judgments and reach conclusions
• Develop and refine practical design and procedures
4
WORD BANK
Below is a list of some key words and phrases you will need to learn and understand in this immobilised
