BIOC 192 – Proteins Worksheet
1. Describe the main differences between primary, secondary, tertiary, and quaternary
protein structures.
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_____________________________________________________________________
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2. Name some ways in which interactions between a molecule and a protein can be
prevented.
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_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
3. What is the effect of lowering pH on protein interactions?
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4. Why are prolines ‘imino acids’ rather than ‘amino acids’?
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5. What is the isoelectric point?
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6. Name some post-translational modifications:
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_______________________________________________________________
_______________________________________________________________
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7. Peptide bonds are: planar/pyramidal, cis/trans, and single/partial double bonds.
,8. State which atoms each of the following bond angles exist between:
Phi: __________________________________________________________
Psi: __________________________________________________________
Omega: _______________________________________________________
Chi: __________________________________________________________
9. What does the Ramachandran plot show?
_____________________________________________________________________
_____________________________________________________________________
10. Label the following diagram with the angles that exist for: antiparallel beta sheets,
parallel beta sheets, right alpha helices, and left alpha helices.
11. What is the usual angle for the omega bond? ___________
12. Describe the interactions found in alpha helices that stabilise the helix.
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
13. Which amino acids are not usually found in alpha helices? Why is this?
_____________________________________________________________________
, _____________________________________________________________________
_____________________________________________________________________
14. Describe the structure of beta sheets.
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
15. What are supersecondary structures?
_____________________________________________________________________
_____________________________________________________________________
16. What are domains/motifs?
_____________________________________________________________________
_____________________________________________________________________
17. Name and give examples of the three main types of domain.
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
18. What is the function of chaperones and chaperonins?
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
19. Name some conditions under which denaturation can occur.
_____________________________________________________________________
_____________________________________________________________________
20. What is the difference between prions and amyloids?
_____________________________________________________________________
_____________________________________________________________________
21. What are the two main categories of post-translational modifications?
_____________________________________________________________________
1. Describe the main differences between primary, secondary, tertiary, and quaternary
protein structures.
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
2. Name some ways in which interactions between a molecule and a protein can be
prevented.
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
3. What is the effect of lowering pH on protein interactions?
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
4. Why are prolines ‘imino acids’ rather than ‘amino acids’?
_____________________________________________________________________
_____________________________________________________________________
5. What is the isoelectric point?
_____________________________________________________________________
_____________________________________________________________________
6. Name some post-translational modifications:
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
7. Peptide bonds are: planar/pyramidal, cis/trans, and single/partial double bonds.
,8. State which atoms each of the following bond angles exist between:
Phi: __________________________________________________________
Psi: __________________________________________________________
Omega: _______________________________________________________
Chi: __________________________________________________________
9. What does the Ramachandran plot show?
_____________________________________________________________________
_____________________________________________________________________
10. Label the following diagram with the angles that exist for: antiparallel beta sheets,
parallel beta sheets, right alpha helices, and left alpha helices.
11. What is the usual angle for the omega bond? ___________
12. Describe the interactions found in alpha helices that stabilise the helix.
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
13. Which amino acids are not usually found in alpha helices? Why is this?
_____________________________________________________________________
, _____________________________________________________________________
_____________________________________________________________________
14. Describe the structure of beta sheets.
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
15. What are supersecondary structures?
_____________________________________________________________________
_____________________________________________________________________
16. What are domains/motifs?
_____________________________________________________________________
_____________________________________________________________________
17. Name and give examples of the three main types of domain.
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
18. What is the function of chaperones and chaperonins?
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________________________________
19. Name some conditions under which denaturation can occur.
_____________________________________________________________________
_____________________________________________________________________
20. What is the difference between prions and amyloids?
_____________________________________________________________________
_____________________________________________________________________
21. What are the two main categories of post-translational modifications?
_____________________________________________________________________
