Microbiology LAB Final Exam LSUE Exam Questions
With 100% Correct Answers.
Cotton plugs or screw caps are used in test tubes containing sterile media or pure cultures to
a. Prevent unfiltered air or dust particles from entering the tubes, maintain anaerobic conditions and
prevent the culture from spilling
The pH indicator in Simmon's citrate agar is what?
a. Bromthymol Blue
Bacteria that show no preference, grow equally well in aerobic or anaerobic conditions are
a. Facultative
The protein that DOES NOT give milk its white color is
a. Albumin
Beef extract is a source of
Salt, Vitamins, Carbohydrates
Bacteria which grow in the dark & in media composed mainly of organic compounds are
Heterotrophic
Media that allows one type of bacteria to grow on them while preventing growth of other types of
bacteria is called
Selective media
If the pH of the medium is higher than 6.8 you can adjust to 6.8 by
Adding drops of HCL to lower it
A medium which contains substances whose exact chemical composition is known is called
Synthetic medium
A microorganism which grows in the presence of very low concentration of free oxygen is known as
Microaerophile
What is the most nearly true of heterotrophs compared to autotrophs?
Heterotrophs have greater synthetic ability
The ability of organisms to hydrolyze carbohydrate accomplished with the enzyme is
Amylase
If a microbe produces gas when it utilizes glucose, the presence of this gas can be determined by
, The use of a Durham tube
A fluid thioglycollate medium is a medium that determines the ____________ of bacteria
Oxygen's requirements
When talking about the VP test, Butanediol fermentation takes places and acetoin is one of the
products formed. What is its chemical nomenclature of an acetoin end product?
2, 3-butanediol
What can you detect from the SIM test?
If the bacteria inhibits motility, Can produce sulfate, Form indole, There is no such thing as a SIM test
Lactose fermentation is indicated on a phenol red lactose medium, turning a _________ color.
Yellow
What is the action of breaking down protein?
Proteolysis
What problem could occur if we made all inoculations for the single tube given to us?
Contamination, Aging the culture, Microbes in the broth culture may die
Yeast broth is a combination of
Yeast extract & nutrient broth
In general, all cellular activities are mediated by which of the following?
Enzymes
Organisms that can use CO2 as their sole or principle source of carbon are
Autotrophs
Media containing some ingredients of unknown chemical composition are called ____ media
Complex media
Growth media that are supplemented by rich nutrient sources in order to support the growth of
organisms with fastidious culture requirements are called __________ media
Which of the following can be used to isolate pure cultures of bacteria from mixtures?
Spread plate, Streak plate, Pour plate
__________ are the compounds most widely used by fermenting organisms. *Fermenting organisms
use sugar
Sugar (glucose)
The solidarity material used in solid or semisolid media is
With 100% Correct Answers.
Cotton plugs or screw caps are used in test tubes containing sterile media or pure cultures to
a. Prevent unfiltered air or dust particles from entering the tubes, maintain anaerobic conditions and
prevent the culture from spilling
The pH indicator in Simmon's citrate agar is what?
a. Bromthymol Blue
Bacteria that show no preference, grow equally well in aerobic or anaerobic conditions are
a. Facultative
The protein that DOES NOT give milk its white color is
a. Albumin
Beef extract is a source of
Salt, Vitamins, Carbohydrates
Bacteria which grow in the dark & in media composed mainly of organic compounds are
Heterotrophic
Media that allows one type of bacteria to grow on them while preventing growth of other types of
bacteria is called
Selective media
If the pH of the medium is higher than 6.8 you can adjust to 6.8 by
Adding drops of HCL to lower it
A medium which contains substances whose exact chemical composition is known is called
Synthetic medium
A microorganism which grows in the presence of very low concentration of free oxygen is known as
Microaerophile
What is the most nearly true of heterotrophs compared to autotrophs?
Heterotrophs have greater synthetic ability
The ability of organisms to hydrolyze carbohydrate accomplished with the enzyme is
Amylase
If a microbe produces gas when it utilizes glucose, the presence of this gas can be determined by
, The use of a Durham tube
A fluid thioglycollate medium is a medium that determines the ____________ of bacteria
Oxygen's requirements
When talking about the VP test, Butanediol fermentation takes places and acetoin is one of the
products formed. What is its chemical nomenclature of an acetoin end product?
2, 3-butanediol
What can you detect from the SIM test?
If the bacteria inhibits motility, Can produce sulfate, Form indole, There is no such thing as a SIM test
Lactose fermentation is indicated on a phenol red lactose medium, turning a _________ color.
Yellow
What is the action of breaking down protein?
Proteolysis
What problem could occur if we made all inoculations for the single tube given to us?
Contamination, Aging the culture, Microbes in the broth culture may die
Yeast broth is a combination of
Yeast extract & nutrient broth
In general, all cellular activities are mediated by which of the following?
Enzymes
Organisms that can use CO2 as their sole or principle source of carbon are
Autotrophs
Media containing some ingredients of unknown chemical composition are called ____ media
Complex media
Growth media that are supplemented by rich nutrient sources in order to support the growth of
organisms with fastidious culture requirements are called __________ media
Which of the following can be used to isolate pure cultures of bacteria from mixtures?
Spread plate, Streak plate, Pour plate
__________ are the compounds most widely used by fermenting organisms. *Fermenting organisms
use sugar
Sugar (glucose)
The solidarity material used in solid or semisolid media is
