Samenvatting Signaaltransductie (Celbiologie) UvA

Samenvatninsiinaaltra ansducte




- Siinaalnisnspecifeen fnden eceptra nisnspecifee
- T ansductenisnnietranalleennhetrand ievennvanneennsiinaalnmaa neann enhetranve strae eennzijnnvan
eennsiinaal
- Ve schillendensiinalenne mennsamenn mneennbeslissinintraennemennweleeneffector prrotens n
m etraennactve en
- Siinaalnm etranw denneunnenniestra ptrand nfeedbacemechanismenn fnd datranden
c mp nentraennmaa neennbepaaldenlevensduu nhebbenn(inieb uwdninnhetraneiwitrandatranjen
actvee trae
- Sngsales
o amin nacids;npeptdes;np traeinsn(ilutraamatrae;nfMLP;nEGFe
o h m nes,ni wtrahnfactra sn(e.i.nstrae ids,nEGFe
o suia sn(iluc see
o nucle tdesn(ATP;nGTP;ncAMP;ncGMPe
o iassesn(NO;netrahylenen(phytra h m neee
o i nsn(Ca2+,nH+en-nfatras/lipidsn(strae ids,np straailandines,nleue tra ienes,n
ph sph in sitdese
o nutra ientrasn(iluc se,namin nacids,nfatrase
o traempe atrau en(c ld,nheatrae
o watrae n(d uihtrae
o sm tcn(i ns,nwatrae np traental,ntrau i e
o mechanicn(tra uch,nstra etrache
o liihtran(=nn trananm lecule;nph tra n,nelectra mainetcn adiat ne
o stra ess
 extra acellula n<->nintra acellula
 l nin<->nsh tranlived
 fastran<->nsl w

,- Stapr 1: receprtores
o Pe ceptenvannhetransiinaal
o 4ncatraei ieënn
 Costact-depresdest = Di ectranc ntraactrantraussenntraweencellen
 l eaal
 Immuunncellen,nemb y ncellaienn
 Gapr Jusctios nall wnneiihb inincellsntra nsha ensiinallininInf nandntra n
esp ndninnanc dinatraednway
o Beatnin fntrahenhea tra
 Paracrnse = L eaalneffectranvanneennuitraiescheidennsiinaaln pn mliiiendencellen
 L eaal
 altrah uihncellsnmayn esp ndntra ntrahei n wnnsiinalsn=nautocrnse
ngsallnsg;ne.i.nmanyncance ncells

,  V beeld:nLacen fn xyienntra iiie sntrahensec et nn fnVEGFn=nvascula n
end trahelialni wtrahnfactra ,nstmulatninasgnogese n (v mininvann
nieuwenbl edvatraene
 G eifactra ennz alsnEpide malni wtrahnfactra n(EDGFenennVascula n
end trahelialni wtrahnfactra (VEGFe,nCytra eines,nStestra fn xiden(NOe,n
end cannabin ïden,nATPn fnRNA
 Sysaprtic = Neu tra ansmitte nafaneelijensiinaalnnaa nande encellen
 Eve ynneu nnc nsistrasn fnancell bodynwitrahntrahennucleusnandnseve aln
b anches:nusuallyn nenlosg axosntrahatranc nductrasntrahensiinalnawayninn
va i usndesdrnte ,np vidininannenla iednsu facena eantra n eceiven
siinalsnf mntrahenax nsn fn trahe nneu ns
 Whennactvatraednbynstmulinf mntrahenenvi nmentran nf mn trahe n
ne vencells,ntrahenneu nnsendsnelectra icalnimpulsesn(act nnp traentalsen
apidlynal ninitrasnax n;nwhennsuchnanninpulsen eachesntrahensynapsnatran
trahenendn fntrahenax n,nitrantra iiie sntrahensec et nn fnanchemicalnsiinalntrahatra
actrasnasnanneu tra ansmitte
 Esdocrnse = Systraemischneffectranvanneennuitraiescheidennsiinaaln pncellenninn
ande enweefselsn(h m nenenviandenbl eds ml p
 Ad enalinen•nC ts ln•nGlucai nn•nInsulinen•nTestra strae nn•n
Schildelie h m nn•nVas p essine
- Perceprtie
o Cell- urface receprtor : Receptra n ntravanitransiinaalnaanndenbuitraeneantranvanndencel:n
Plasmamemb aann(hydrofiele moleculese
o Istracellular receprtor : Receptra n ntravanitransiinaalnbinnenninndencel:nCytra plasma,n
Memb aanstra uctrau enn fnKe nn(hydrofobe moleculese
 Small hydroprhobnc ngsal molecule ncanndiffusendi ectralynac ssntrahenplasman
memb ane.nE.i.nstrae idnh m nes,ntrahy idnh m nes,n etn ids,nvitraaminnD
 Theyna enallndiffe entranbutranactranvianansimila nmechanism,nandna enpa tran fnan
suclear receprtor uprerfamnly.




- Then ame cell cann eactrantra ndnfferest ngsal ,tra iiie inindnfferest re pros e n
o V beeld:nacetylcholnse

, - Eachncellnisnp i ammedntra n esp ndntra nspecifcnc mbinat nsn fnextra acellula nsiinals
- Celln equi enmultplensiinalsntra n urvnve,n
- andnaddit nalnsiinalsntra ngrow asd devnde,n ntra ndnfferestiate.n
- Ifndep ivedn fnapp p iatraensu vivalnsiinals,nancellnwillnunde i nanf mn fnsuicide,nen wnnasn
Aproprto n n
o N trae:nThenactraualnsitrauat nnisnm enc mplex,nasns mensiinalsnactrantra ninhibitran
(antraai niseen ninducenap ptra sis
- Then ame ngsalncanntra iiie ndnfferest re pros e ninntrahen ame cell n
o Thenfatraen fns mendevel pinincellsndependsn nnthe coscestratios of a morprhoges
o Anmorprhogesncannbenanpeptden np traeinnsiinalnbutraninnplantrasnals nchemical,ni.e.ntrahen
phytra h m ne,nauxin
o Diffe entranc ncentra at nsn fnm ph ienninducentrahenexp essi nn fndiffe entransetrasn fn
ienes,n esultnininndiffe entrancellnfatraesnandndevel pmentra




- Ancellncannaltrae ntrahenc ncentra at nn fnannintra acellula nm leculenquicely,nifntrahenlifetmen fntrahen
m leculenisnsh tra
- The suclear receprtor uprerfamnly
o INACTIVEnRECEPTORn
 Allnnuclea n eceptra snbindntra nDNAnasneitrahe nh m dime sn nhetrae dime s.nF
simplicitray,ntraheyna ensh wnnasnm n me snhe e
 Stra uctrau e:nDNA-bnsdnsg domans, lngasd-bnsdnsg domans asd tras crnprtios-
activatisg domans
 Then eceptra nisnINACTIVEnbecausenannnshnbntory prrotensnisnb und
o RECEPTORnACTIVATION
 Bindinin fntrahenliiandntra ntrahen eceptra ,ncauses:n
 lngasd-bnsdnsg domansn fntrahen eceptra ntra nclampnshutrana undntrahen
liiand
 'inhibitra ynp traein'ntra ndiss ciatrae