BCH 205
Introductory molecular biology
NUCLEASES
DR H.A. BANKOLE
, Introduction
Stuart Linn and Werner Arber isolated two types of enzymes in 1960s in phage responsible fo
their restriction growth in E. coli
One of the enzyme added a methyl group to the DNA generating a methylated DNA, while th
other enzyme cleaved an unmethylated DNA at different locations along the length of the DN
The first enzyme was called a methylase, while the other was called a restriction nuclease
The first restriction nuclease whose function is dependent on specific DNA nucleotide sequen
was isolated and characterized in 1968 by H.O. Smith, K.W. Wilcox, and T.J. Kelley from
Haemophilus infuenza bacteria
The enzyme isolated (HindII) will always cut DNA molecule at a particular point within a spec
sequence of six base pairs
Introductory molecular biology
NUCLEASES
DR H.A. BANKOLE
, Introduction
Stuart Linn and Werner Arber isolated two types of enzymes in 1960s in phage responsible fo
their restriction growth in E. coli
One of the enzyme added a methyl group to the DNA generating a methylated DNA, while th
other enzyme cleaved an unmethylated DNA at different locations along the length of the DN
The first enzyme was called a methylase, while the other was called a restriction nuclease
The first restriction nuclease whose function is dependent on specific DNA nucleotide sequen
was isolated and characterized in 1968 by H.O. Smith, K.W. Wilcox, and T.J. Kelley from
Haemophilus infuenza bacteria
The enzyme isolated (HindII) will always cut DNA molecule at a particular point within a spec
sequence of six base pairs
