QBM Final Exam Review|Complete Questions
with A+ Graded Answers
What is transcription?
DNA > RNA
What is the best representation/model of proteins?
Molecular Surface Model
Describe the four levels of protein structure.
Primary: amino acid sequence
Secondary: the primary chain forms spirals(alpha helix) and sheets(beta sheets)
Tertiary: 3-D with folded motifs
Quaternary structure: Two or more polypeptide chains, with its own tertiary structure, combine to
form a functional protein.
What does OSHA stand for?
Occupational Safety and Health Administration
What is the role of OSHA?
employers must inform employees of potential hazards
What is included in the MSDS?
Chemical's physical and chemical properties, toxic, carcinogenic etc.
and describe the proper way of handling these chemicals
Describe the four biosafety levels
BSL-1: Minimal hazards.
BSL-2: Organisms with potential hazards; proper protective equipment; waste disposed properly;
most teaching labs and some research labs. (EX:QBM lab)
BSL-3: Pathogenic organisms that cause serious disease; proper ventilation; lab restriction; special
protective equipment; some research labs
BSL-4: Dangerous pathogens that cause severe to fatal disease; no vaccines or treatments
List the from best to worst glove type
Latex > Nitrile > Plastic > None
how much should be used when using graduated cylinder?
25ml-2L
What is a transfer/pasteur pipette used for?
quick measurements, non-accurate
What is a micropipette used for?
, 0.1 ul to 1 ml accuracy, the tip in the 1 cm of liquid and held vertically.
Describe an autoclave
Device with high temperatures and pressure used to sterilize glassware and peptides
Describe centrifugation
used to separate samples based on mass, shape and density
Describe a fixed angle rotor?
most common, particles pellet to a side when centrifuged
Describe a swinging bucket rotor?
does NOT creat tight pellets, for sample analysis or running cesium chloride or sucrose density
gradients. Used to separate by density.
Describe clear (polycarbonate) bottles
Better visibility, less resistant to chemicals, can weaken/crack
Describe opaque (polypropylene) bottles
More resistant to chemicals and cracks
Describe Spectroscopy
study of radiation interacting with matter, can analyze molecular structure (can be electromagnetic or
non-electromagnetic)
Describe spectrometry
Measurement of the spectroscopy interactions allowing to determine molecular structure &
concentrations
Describe a Spectrophotometer
measures the intensity of a specific wavelength as it passes through a sample and compares it to a
blank
what are the components of a spectrophotometer
light source(tungsten or deuterium), monochromator, prism, sample compartment, detector
Describe plate readers
quickly read ul volumes pf hundreds of samples from a single plate (used in ELISA)
How to determine DNA purity
A260/A280 ratio > 1.5 = pure
what is the equation to calculate absorbance and transmittance?
· A=-log T (where T is a decimal), T=10^A.
with A+ Graded Answers
What is transcription?
DNA > RNA
What is the best representation/model of proteins?
Molecular Surface Model
Describe the four levels of protein structure.
Primary: amino acid sequence
Secondary: the primary chain forms spirals(alpha helix) and sheets(beta sheets)
Tertiary: 3-D with folded motifs
Quaternary structure: Two or more polypeptide chains, with its own tertiary structure, combine to
form a functional protein.
What does OSHA stand for?
Occupational Safety and Health Administration
What is the role of OSHA?
employers must inform employees of potential hazards
What is included in the MSDS?
Chemical's physical and chemical properties, toxic, carcinogenic etc.
and describe the proper way of handling these chemicals
Describe the four biosafety levels
BSL-1: Minimal hazards.
BSL-2: Organisms with potential hazards; proper protective equipment; waste disposed properly;
most teaching labs and some research labs. (EX:QBM lab)
BSL-3: Pathogenic organisms that cause serious disease; proper ventilation; lab restriction; special
protective equipment; some research labs
BSL-4: Dangerous pathogens that cause severe to fatal disease; no vaccines or treatments
List the from best to worst glove type
Latex > Nitrile > Plastic > None
how much should be used when using graduated cylinder?
25ml-2L
What is a transfer/pasteur pipette used for?
quick measurements, non-accurate
What is a micropipette used for?
, 0.1 ul to 1 ml accuracy, the tip in the 1 cm of liquid and held vertically.
Describe an autoclave
Device with high temperatures and pressure used to sterilize glassware and peptides
Describe centrifugation
used to separate samples based on mass, shape and density
Describe a fixed angle rotor?
most common, particles pellet to a side when centrifuged
Describe a swinging bucket rotor?
does NOT creat tight pellets, for sample analysis or running cesium chloride or sucrose density
gradients. Used to separate by density.
Describe clear (polycarbonate) bottles
Better visibility, less resistant to chemicals, can weaken/crack
Describe opaque (polypropylene) bottles
More resistant to chemicals and cracks
Describe Spectroscopy
study of radiation interacting with matter, can analyze molecular structure (can be electromagnetic or
non-electromagnetic)
Describe spectrometry
Measurement of the spectroscopy interactions allowing to determine molecular structure &
concentrations
Describe a Spectrophotometer
measures the intensity of a specific wavelength as it passes through a sample and compares it to a
blank
what are the components of a spectrophotometer
light source(tungsten or deuterium), monochromator, prism, sample compartment, detector
Describe plate readers
quickly read ul volumes pf hundreds of samples from a single plate (used in ELISA)
How to determine DNA purity
A260/A280 ratio > 1.5 = pure
what is the equation to calculate absorbance and transmittance?
· A=-log T (where T is a decimal), T=10^A.
