QBM Final Exam Practice
Which of the following is LEAST suited to analyze titin, a large protein?
MS
Bioinformatics
All answer choices are correct.
In flow cytometry, the forward scattering of light can indicate __________, and the side scatter
indicates __________.
the size of a cell; the cell's granularity, or health.
Mass spectrometers can have some variation in peak shape due to
isotopes.
__________ can be used for the resolution of individual atoms. This is performed by __________.
Cryo-EM; many copies of the sample are frozen and placed under an electron microscope.
A knockin mouse
has a gene insertion.
Which of the following techniques would be best suited to directly study cellular metabolism in vitro?
ExPASy
Sequence analysis
All answer choices are correct.
BLAST stands for
Basic Local Alignment Search Tool.
Which of the following best describes the Cre-Lox system?
A transgenic mouse that knocks out a target gene only in a specific cell type, by utilizing gene
inversion.
A __________ can be used to predict the secondary structures of proteins.
Ramachandran plot
The final step of X-ray crystallography is to
calculate an electron density map.
Which of the following best describes shotgun sequencing?
Genome is sheared, ligated into plasmids via TA cloning, sequenced with primers, and pieced together
on a computer.
Which of the following is LEAST suited to analyze titin, a large protein?
MS
Bioinformatics
All answer choices are correct.
In flow cytometry, the forward scattering of light can indicate __________, and the side scatter
indicates __________.
the size of a cell; the cell's granularity, or health.
Mass spectrometers can have some variation in peak shape due to
isotopes.
__________ can be used for the resolution of individual atoms. This is performed by __________.
Cryo-EM; many copies of the sample are frozen and placed under an electron microscope.
A knockin mouse
has a gene insertion.
Which of the following techniques would be best suited to directly study cellular metabolism in vitro?
ExPASy
Sequence analysis
All answer choices are correct.
BLAST stands for
Basic Local Alignment Search Tool.
Which of the following best describes the Cre-Lox system?
A transgenic mouse that knocks out a target gene only in a specific cell type, by utilizing gene
inversion.
A __________ can be used to predict the secondary structures of proteins.
Ramachandran plot
The final step of X-ray crystallography is to
calculate an electron density map.
Which of the following best describes shotgun sequencing?
Genome is sheared, ligated into plasmids via TA cloning, sequenced with primers, and pieced together
on a computer.
