QBM Practice Exam 4 ( Questions And
Answers) Verified 100% Correct!!
Which of the following expression systems is fastest, but would not glycosylate a protein?
Bacteria
Transfection
can be performed using the capabilities of retroviruses.
__________ is a form of post-transcriptional gene regulation in which non-translated double-stranded
RNA (dsRNA) molecules called small interfering RNA (siRNA) mediate sequence specific degradation of
target messenger RNA (mRNA).
RNA interference
Cells can be directly modified via __________, which involves using a small glass pipette or
microscopic lance to add or remove nuclear material (enucleation) or inject molecules into a cell,
through the plasma membrane.
microinjection
Immunofluorescence is most similar to performing
a western blot.
Which of the following techniques would be best suited to directly study cellular metabolism in vitro?
Pulse-chase.
In FACS, cells can be separated based on
stage of cell cycle.
size.
health.
number of chromosomes.
In flow cytometry, the forward scattering of light can indicate __________, and the side scatter
indicates __________.
the size of a cell ; the cell's granularity, or health
During in situ organ perfusion,
an organ is removed from the model organism, maintained at constant temperature and pH in an
apparatus, and fluid is passed through the organ.
Which of the following is a benefit of lower-order model organisms versus higher-order model
organisms?
Short development time.
Any investigator wishing to use animals in a research program must first submit a detailed animal
protocol to __________ for approval.
the IACUC committee
, Which of the following statements concerning transgenic animals is correct?
Pigs with human-sized organs have been designed, to aid in the supply of transplant organs.
The incorporation of GFP into the genome of animals results in the creation of a transgenic animal.
Transgenic animals have been used to make model organisms for human illnesses, by mimicking
phenotypes.
Transgenic animals have non-native genomes, or genomes that have been modified.
(ALL)
RNAi can only be used in cell culture, not in vivo
False
Cloning
NOT used to involve iPSCs, but now is mostly performed directly.
NOT is now performed by inserting the nucleus of the cell to be cloned into the uterus directly.
What is the benefit of using knockdown mice instead of knockout mice?
The mutation may be embryonic lethal; knockdown would allow to still assess the effects of the gene.
A knock-in mouse
has a gene insertion.
Which of the following best describes the Cre-Lox system?
A transgenic mouse that knocks out a target gene only in a specific cell type, by utilizing gene
inversion.
Which of the following either can be performed now or may be possible in the future using CRISPR?
Repair diseases caused by point mutations.
Bring the wooly mammoth back from extinction.
Cure lung cancer by editing T cells.
Create animal models by knocking out or knocking down genes.
(ALL)
Optogenetics can be used to
transfect brain cells with channelrhodopsin, causing the cells to be controllable by light activation.
Which of the following is used for lab mice that undergo procedures with lasting pain effects?
Painkillers
Bioinformatics
can incorporate mathematics, informatics, statistics, computer science, artificial intelligence,
chemistry, and biochemistry.
techniques can be referred to as in silico techniques.
includes the analysis of genomes, genes, and proteins using computer algorithms and computer
databases.
is the computational branch of molecular biology.
(ALL)
Answers) Verified 100% Correct!!
Which of the following expression systems is fastest, but would not glycosylate a protein?
Bacteria
Transfection
can be performed using the capabilities of retroviruses.
__________ is a form of post-transcriptional gene regulation in which non-translated double-stranded
RNA (dsRNA) molecules called small interfering RNA (siRNA) mediate sequence specific degradation of
target messenger RNA (mRNA).
RNA interference
Cells can be directly modified via __________, which involves using a small glass pipette or
microscopic lance to add or remove nuclear material (enucleation) or inject molecules into a cell,
through the plasma membrane.
microinjection
Immunofluorescence is most similar to performing
a western blot.
Which of the following techniques would be best suited to directly study cellular metabolism in vitro?
Pulse-chase.
In FACS, cells can be separated based on
stage of cell cycle.
size.
health.
number of chromosomes.
In flow cytometry, the forward scattering of light can indicate __________, and the side scatter
indicates __________.
the size of a cell ; the cell's granularity, or health
During in situ organ perfusion,
an organ is removed from the model organism, maintained at constant temperature and pH in an
apparatus, and fluid is passed through the organ.
Which of the following is a benefit of lower-order model organisms versus higher-order model
organisms?
Short development time.
Any investigator wishing to use animals in a research program must first submit a detailed animal
protocol to __________ for approval.
the IACUC committee
, Which of the following statements concerning transgenic animals is correct?
Pigs with human-sized organs have been designed, to aid in the supply of transplant organs.
The incorporation of GFP into the genome of animals results in the creation of a transgenic animal.
Transgenic animals have been used to make model organisms for human illnesses, by mimicking
phenotypes.
Transgenic animals have non-native genomes, or genomes that have been modified.
(ALL)
RNAi can only be used in cell culture, not in vivo
False
Cloning
NOT used to involve iPSCs, but now is mostly performed directly.
NOT is now performed by inserting the nucleus of the cell to be cloned into the uterus directly.
What is the benefit of using knockdown mice instead of knockout mice?
The mutation may be embryonic lethal; knockdown would allow to still assess the effects of the gene.
A knock-in mouse
has a gene insertion.
Which of the following best describes the Cre-Lox system?
A transgenic mouse that knocks out a target gene only in a specific cell type, by utilizing gene
inversion.
Which of the following either can be performed now or may be possible in the future using CRISPR?
Repair diseases caused by point mutations.
Bring the wooly mammoth back from extinction.
Cure lung cancer by editing T cells.
Create animal models by knocking out or knocking down genes.
(ALL)
Optogenetics can be used to
transfect brain cells with channelrhodopsin, causing the cells to be controllable by light activation.
Which of the following is used for lab mice that undergo procedures with lasting pain effects?
Painkillers
Bioinformatics
can incorporate mathematics, informatics, statistics, computer science, artificial intelligence,
chemistry, and biochemistry.
techniques can be referred to as in silico techniques.
includes the analysis of genomes, genes, and proteins using computer algorithms and computer
databases.
is the computational branch of molecular biology.
(ALL)
