QBM Final Exam Practice
Which of the following is LEAST suited to analyze titin, a large protein?
MS
Bioinformatics
All answer choices are correct.
In flow cytometry, the forward scattering of light can indicate __________, and the
side scatter indicates __________.
the size of a cell; the cell's granularity, or health.
Mass spectrometers can have some variation in peak shape due to
isotopes.
__________ can be used for the resolution of individual atoms. This is performed
by __________.
Cryo-EM; many copies of the sample are frozen and placed under an electron
microscope.
A knockin mouse
has a gene insertion.
Which of the following techniques would be best suited to directly study cellular
metabolism in vitro?
ExPASy
Sequence analysis
All answer choices are correct.
BLAST stands for
Basic Local Alignment Search Tool.
Which of the following best describes the Cre-Lox system?
A transgenic mouse that knocks out a target gene only in a specific cell type, by utilizing
gene inversion.
A __________ can be used to predict the secondary structures of proteins.
Ramachandran plot
The final step of X-ray crystallography is to
calculate an electron density map.
Which of the following best describes shotgun sequencing?
Genome is sheared, ligated into plasmids via TA cloning, sequenced with primers, and
pieced together on a computer.
Genome is sheared, ligated into plasmids via TA cloning, sequenced with
primers, and pieced together on a computer.
Circular dichroism
What is the benefit of using knockdown mice instead of knockout mice?
The mutation may be embryonic lethal; knockdown would allow to still assess the
effects of the gene.
Which of the following either can be performed now or may be possible in the
future using CRISPR?
All answer choices are correct.
Which of the following is NOT a feature of drug design?
Structure-based drug design exhibits an increase in false positives compared to high-
throughput screening.
Which of the following is LEAST suited to analyze titin, a large protein?
MS
Bioinformatics
All answer choices are correct.
In flow cytometry, the forward scattering of light can indicate __________, and the
side scatter indicates __________.
the size of a cell; the cell's granularity, or health.
Mass spectrometers can have some variation in peak shape due to
isotopes.
__________ can be used for the resolution of individual atoms. This is performed
by __________.
Cryo-EM; many copies of the sample are frozen and placed under an electron
microscope.
A knockin mouse
has a gene insertion.
Which of the following techniques would be best suited to directly study cellular
metabolism in vitro?
ExPASy
Sequence analysis
All answer choices are correct.
BLAST stands for
Basic Local Alignment Search Tool.
Which of the following best describes the Cre-Lox system?
A transgenic mouse that knocks out a target gene only in a specific cell type, by utilizing
gene inversion.
A __________ can be used to predict the secondary structures of proteins.
Ramachandran plot
The final step of X-ray crystallography is to
calculate an electron density map.
Which of the following best describes shotgun sequencing?
Genome is sheared, ligated into plasmids via TA cloning, sequenced with primers, and
pieced together on a computer.
Genome is sheared, ligated into plasmids via TA cloning, sequenced with
primers, and pieced together on a computer.
Circular dichroism
What is the benefit of using knockdown mice instead of knockout mice?
The mutation may be embryonic lethal; knockdown would allow to still assess the
effects of the gene.
Which of the following either can be performed now or may be possible in the
future using CRISPR?
All answer choices are correct.
Which of the following is NOT a feature of drug design?
Structure-based drug design exhibits an increase in false positives compared to high-
throughput screening.
