QBM Final Exam Review|Complete Questions with A+
Graded Answers
What is transcription?
DNA > RNA
What is the best representation/model of proteins?
Molecular Surface Model
Describe the four levels of protein structure.
Primary: amino acid sequence
Secondary: the primary chain forms spirals(alpha helix) and sheets(beta sheets)
Tertiary: 3-D with folded motifs
Quaternary structure: Two or more polypeptide chains, with its own tertiary structure,
combine to form a functional protein.
What does OSHA stand for?
Occupational Safety and Health Administration
What is the role of OSHA?
employers must inform employees of potential hazards
What is included in the MSDS?
Chemical's physical and chemical properties, toxic, carcinogenic etc.
and describe the proper way of handling these chemicals
Describe the four biosafety levels
BSL-1: Minimal hazards.
BSL-2: Organisms with potential hazards; proper protective equipment; waste disposed
properly; most teaching labs and some research labs. (EX:QBM lab)
BSL-3: Pathogenic organisms that cause serious disease; proper ventilation; lab
restriction; special protective equipment; some research labs
BSL-4: Dangerous pathogens that cause severe to fatal disease; no vaccines or
treatments
List the from best to worst glove type
Latex > Nitrile > Plastic > None
how much should be used when using graduated cylinder?
25ml-2L
What is a transfer/pasteur pipette used for?
quick measurements, non-accurate
What is a micropipette used for?
0.1 ul to 1 ml accuracy, the tip in the 1 cm of liquid and held vertically.
Describe an autoclave
Device with high temperatures and pressure used to sterilize glassware and peptides
Describe centrifugation
used to separate samples based on mass, shape and density
Describe a fixed angle rotor?
most common, particles pellet to a side when centrifuged
Graded Answers
What is transcription?
DNA > RNA
What is the best representation/model of proteins?
Molecular Surface Model
Describe the four levels of protein structure.
Primary: amino acid sequence
Secondary: the primary chain forms spirals(alpha helix) and sheets(beta sheets)
Tertiary: 3-D with folded motifs
Quaternary structure: Two or more polypeptide chains, with its own tertiary structure,
combine to form a functional protein.
What does OSHA stand for?
Occupational Safety and Health Administration
What is the role of OSHA?
employers must inform employees of potential hazards
What is included in the MSDS?
Chemical's physical and chemical properties, toxic, carcinogenic etc.
and describe the proper way of handling these chemicals
Describe the four biosafety levels
BSL-1: Minimal hazards.
BSL-2: Organisms with potential hazards; proper protective equipment; waste disposed
properly; most teaching labs and some research labs. (EX:QBM lab)
BSL-3: Pathogenic organisms that cause serious disease; proper ventilation; lab
restriction; special protective equipment; some research labs
BSL-4: Dangerous pathogens that cause severe to fatal disease; no vaccines or
treatments
List the from best to worst glove type
Latex > Nitrile > Plastic > None
how much should be used when using graduated cylinder?
25ml-2L
What is a transfer/pasteur pipette used for?
quick measurements, non-accurate
What is a micropipette used for?
0.1 ul to 1 ml accuracy, the tip in the 1 cm of liquid and held vertically.
Describe an autoclave
Device with high temperatures and pressure used to sterilize glassware and peptides
Describe centrifugation
used to separate samples based on mass, shape and density
Describe a fixed angle rotor?
most common, particles pellet to a side when centrifuged
