TURCAN SBB EXAM REVIEW AABB
PACKET {GRADED A+}
Rh inheritence of Rhnull - ✔✔Lack LW
Lack FY5
KEL3 Issues - ✔✔KPa
in cis position may have suppressive effect on other Kell system
antigens
Adsorption applications in RBC testing - ✔✔Remove
autoantibody
Separate multiple antibodies
Confirm antigen or antibody specificity
Antilymphocyte Globulin (ALG) - ✔✔Made from horse serum
Interferes with DAT and IAT
Relation to Lu(a-b-)
,PCR and TMA - ✔✔PCR used for DNA amp
TMA used for RNA amp
Autologous donor qualifications - ✔✔DR's order
Hgb >11, HCT 33%
Collected >72 hrs before surgery or Tx
Deferred if risk of bacteremia
Plateletpheresis count info - ✔✔>150,000 min
pre procedure plt count not required for infrequent donors
post-procedure plt count may be used to qualify donor for next
procedure
Two units RBC apheresis post donation H/H - ✔✔Donor HCT not
<30% or Hgb <10 g/dL after volume replacement following
donation
Anticoagulants and purpose of - ✔✔Dextrose:
Supports ATP generation
Adenine:
Provides substrate for ATP synthesis
, Sodium biphosphate:
Controls pH or is buffer
Citrate:
prevents clotting and coagulation
Changes in bag upon storage - ✔✔decreases in:
pH
2,3-DPG
ATP
Increases in:
Hgb
K
Oxygen dissociation curve information - ✔✔p50 is pO2 level at
which Hgb is 50% saturated
A normal p50 is about 25 pO2
increase p50, see right shift, more O2 released
decrease p50, see left shift, less O2 released
PACKET {GRADED A+}
Rh inheritence of Rhnull - ✔✔Lack LW
Lack FY5
KEL3 Issues - ✔✔KPa
in cis position may have suppressive effect on other Kell system
antigens
Adsorption applications in RBC testing - ✔✔Remove
autoantibody
Separate multiple antibodies
Confirm antigen or antibody specificity
Antilymphocyte Globulin (ALG) - ✔✔Made from horse serum
Interferes with DAT and IAT
Relation to Lu(a-b-)
,PCR and TMA - ✔✔PCR used for DNA amp
TMA used for RNA amp
Autologous donor qualifications - ✔✔DR's order
Hgb >11, HCT 33%
Collected >72 hrs before surgery or Tx
Deferred if risk of bacteremia
Plateletpheresis count info - ✔✔>150,000 min
pre procedure plt count not required for infrequent donors
post-procedure plt count may be used to qualify donor for next
procedure
Two units RBC apheresis post donation H/H - ✔✔Donor HCT not
<30% or Hgb <10 g/dL after volume replacement following
donation
Anticoagulants and purpose of - ✔✔Dextrose:
Supports ATP generation
Adenine:
Provides substrate for ATP synthesis
, Sodium biphosphate:
Controls pH or is buffer
Citrate:
prevents clotting and coagulation
Changes in bag upon storage - ✔✔decreases in:
pH
2,3-DPG
ATP
Increases in:
Hgb
K
Oxygen dissociation curve information - ✔✔p50 is pO2 level at
which Hgb is 50% saturated
A normal p50 is about 25 pO2
increase p50, see right shift, more O2 released
decrease p50, see left shift, less O2 released
