Task 2: How to identify analytes using GC and HPLC
State the key features of a GC chromatogram and what information can be obtained from one.
The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound
The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation
Peak area can be used to know the quantity of compound in a sample. This is useful for analysis
Peak width can indicate if the separation was efficient or not. long and narrow suggest good
separation while broad and short suggest bad separation.
Baseline is the detectors response to no compound. Stable and flat baseline can suggest a clean
chromatogram
State the key features of a HPLC chromatogram and what information can be obtained from one.
The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound
The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation
Peak area can be used to know the quantity of compound in a sample. This is useful for analysis
Peak width can indicate if the separation was efficient or not. ong and narrow suggest good
separation while broad and short suggest bad separation.
Baseline is the detectors response to no compound. Stable and flat baseline can sugest a clean
chromatogram
, GC Chromatograms.
I will be analysing 4 different chromatograms from against a reference chromatogram to determine
the pesticides present, the peak area of each sample and thus the percentage of each analyte
present and the concentration of each analyte in the sample.
Explain how to calculate peak area. Include an example calculation.
To calculate the peak are you must measure the heigh of the peak and multiply it by the half of the
width. it cam be represented as;
Height of peak/ hath of the width of peak
Example
Height is 15
Width is 4
The it will be
7 /2 = 3.5
3.5 is the peak area
Explain how to calculate percentage of each analyte. Include an example calculation.
To calculate the percentage of each analyte, it must be the peak area of the analyte divided by sum
of all the peak area.
It can be represented as
peak area/sum of all the peak area x100
example
you have an beak areas of 34,7.8,15,10,16.8
what you will do is to add up every thing which will give you 83.6
then you use the sum to divide by the individual peak area and multiply by 100
(34/83.6) x100 = 14.67%
Explain how to calculate concentration of each analyte. Include an example calculation
To calculate the concentration of each analyte
State the key features of a GC chromatogram and what information can be obtained from one.
The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound
The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation
Peak area can be used to know the quantity of compound in a sample. This is useful for analysis
Peak width can indicate if the separation was efficient or not. long and narrow suggest good
separation while broad and short suggest bad separation.
Baseline is the detectors response to no compound. Stable and flat baseline can suggest a clean
chromatogram
State the key features of a HPLC chromatogram and what information can be obtained from one.
The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound
The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation
Peak area can be used to know the quantity of compound in a sample. This is useful for analysis
Peak width can indicate if the separation was efficient or not. ong and narrow suggest good
separation while broad and short suggest bad separation.
Baseline is the detectors response to no compound. Stable and flat baseline can sugest a clean
chromatogram
, GC Chromatograms.
I will be analysing 4 different chromatograms from against a reference chromatogram to determine
the pesticides present, the peak area of each sample and thus the percentage of each analyte
present and the concentration of each analyte in the sample.
Explain how to calculate peak area. Include an example calculation.
To calculate the peak are you must measure the heigh of the peak and multiply it by the half of the
width. it cam be represented as;
Height of peak/ hath of the width of peak
Example
Height is 15
Width is 4
The it will be
7 /2 = 3.5
3.5 is the peak area
Explain how to calculate percentage of each analyte. Include an example calculation.
To calculate the percentage of each analyte, it must be the peak area of the analyte divided by sum
of all the peak area.
It can be represented as
peak area/sum of all the peak area x100
example
you have an beak areas of 34,7.8,15,10,16.8
what you will do is to add up every thing which will give you 83.6
then you use the sum to divide by the individual peak area and multiply by 100
(34/83.6) x100 = 14.67%
Explain how to calculate concentration of each analyte. Include an example calculation
To calculate the concentration of each analyte
