BIOL 2230 Lab Midterm Questions and Answers

BIOL 2230 Lab Midterm Questions and Answers Scientific Method - Make Observations Ask Questions Form a Hypothesis Collect Data Controls: Positive and Negative Draw logical Conclusions Repeat experiments Share your findings Get Feedback Revise hypotheses and statements Form New questions, ideas, hypotheses, and experiments Agar vs Broth - Agar allow organisms to be grown on top Broth allows organisms to be grown within What are the types of media? - Complex Reducing Enriched Selective Differential Defined Complex Media - Contains basic nutrients needed for growth of most bacteria and fungi Reducing Media - Contains basic nutrients needed for growth of most bacteria, plus ingredients which will react with and remove dissolved oxygen. Used for growth of obligate anaerobes. Most are incubated in an oxygen free environment. Enriched Media - Contains basic nutrients for growth, plus special added ingredients (ex: blood, vitamins, etc.). Used for growth of fastidious (hard to grow) microorganisms. Selective Media - Supports the growth of one type of medium and holds down the growth of another. Differential Media - Contain basic nutrients for growth, plus ingredients which allow for visual discrimination between different types of microorganisms. Defined Media - Contains exact, known amounts of all nutrients present. Capnophiles - Require extra carbon dioxide for optimal growth Anaerobe Jar - Used to grow fastidious organisms Example of Short-term Storage - Refrigeration Examples of Long-term Storage - Freezing Deep-freezing Freeze-drying (lyophilization) Measuring Bacterial Growth - Live cell count: Plate count Filtration Metabolic activity: -production of wastes