BIOS 242 Lab Practical Exam With
Correct Questions And Answers 2024
can grow aerobically and anaerobically - CORRECT ANSWERS-S. marcescens
why is proper aseptic technique important? - CORRECT ANSWERS-To prevent
contamination from outside sources
whats the point of flaming the inoculating loop? - CORRECT ANSWERS-heats it up to
the point that if there was any bacteria it would be killed
what happens if you put the hot loop into the tube too soon? - CORRECT ANSWERS-it
might kill the bacteria you are trying to retrieve
why do you stab the agar? - CORRECT ANSWERS-to penetrate the agar to allow for
bacteria to grow anaerobically
what happens if there is a breakdown in aseptic technique? - CORRECT ANSWERS-
breakout of a disease
shape of s. marcescens - CORRECT ANSWERS-circular
shape of e. coli - CORRECT ANSWERS-circular
elevation of s. marcescens - CORRECT ANSWERS-umbonate
why do you streak the four quadrants? - CORRECT ANSWERS-limit the number of
bacteria in each quadrant (isolate colonies)
magnification of objective lens x magnification of ocular lens - CORRECT ANSWERS-
total magnification
20x objective and 10x eyepiece - CORRECT ANSWERS-200x
50x objective x 15x eyepiece - CORRECT ANSWERS-750x
acid fast primary stain - CORRECT ANSWERS-carbolfuchsin
used for organisms that don't stain well with gram due to the waxy outer layer -
CORRECT ANSWERS-acid fast stain
what does increasing magnification do to field of vision? - CORRECT ANSWERS-
increased magnification decreases field of vision
, why is oil immersion necessary at 100x? - CORRECT ANSWERS-more light is going
through your slide thus making it a clearer image
when do you use oil immersion? - CORRECT ANSWERS-100x
means that you can change the focus (objective lens) of a microscope without having to
focus it every time - CORRECT ANSWERS-parfocal
how do you change the light reaching the ocular lens? - CORRECT ANSWERS-light
under the stage or the iris diaphragm
controls the amount of light that is passing through (the larger the hole the more light) -
CORRECT ANSWERS-iris diaphragm
single celled - CORRECT ANSWERS-protozoa
do you need oil immersion for eukaryotes? - CORRECT ANSWERS-no because
eukaryotes are so big you do not need to magnify them beyond 40x
gram positive rod - CORRECT ANSWERS-b. cereus
responsible for making beer - CORRECT ANSWERS-s. cerveisiae
why do you heat fix a specimen? - CORRECT ANSWERS-to attach the bacteria to the
slide so it doesn't wash off during the staining process
why do you use a wet mount? - CORRECT ANSWERS-the water helps to improve
clarity of the organism also if it was a live organism you would be able to see it move
why do we use simple staining techniques? - CORRECT ANSWERS-allows us to see
size, morphology, and arrangement
why do you air dry your slide? - CORRECT ANSWERS-it makes sure that the bacteria
is dry so when you heat it it doesn't change the shape of the bacteria
gram positive bacteria (cocci) - CORRECT ANSWERS-s. epidermidis
purple - CORRECT ANSWERS-gram positive
pink - CORRECT ANSWERS-gram negative
thick layer of peptidoglycn - CORRECT ANSWERS-gram positive
thin layer of peptidoglycan with an outer membrane that contains lipid a - CORRECT
ANSWERS-gram negative
Correct Questions And Answers 2024
can grow aerobically and anaerobically - CORRECT ANSWERS-S. marcescens
why is proper aseptic technique important? - CORRECT ANSWERS-To prevent
contamination from outside sources
whats the point of flaming the inoculating loop? - CORRECT ANSWERS-heats it up to
the point that if there was any bacteria it would be killed
what happens if you put the hot loop into the tube too soon? - CORRECT ANSWERS-it
might kill the bacteria you are trying to retrieve
why do you stab the agar? - CORRECT ANSWERS-to penetrate the agar to allow for
bacteria to grow anaerobically
what happens if there is a breakdown in aseptic technique? - CORRECT ANSWERS-
breakout of a disease
shape of s. marcescens - CORRECT ANSWERS-circular
shape of e. coli - CORRECT ANSWERS-circular
elevation of s. marcescens - CORRECT ANSWERS-umbonate
why do you streak the four quadrants? - CORRECT ANSWERS-limit the number of
bacteria in each quadrant (isolate colonies)
magnification of objective lens x magnification of ocular lens - CORRECT ANSWERS-
total magnification
20x objective and 10x eyepiece - CORRECT ANSWERS-200x
50x objective x 15x eyepiece - CORRECT ANSWERS-750x
acid fast primary stain - CORRECT ANSWERS-carbolfuchsin
used for organisms that don't stain well with gram due to the waxy outer layer -
CORRECT ANSWERS-acid fast stain
what does increasing magnification do to field of vision? - CORRECT ANSWERS-
increased magnification decreases field of vision
, why is oil immersion necessary at 100x? - CORRECT ANSWERS-more light is going
through your slide thus making it a clearer image
when do you use oil immersion? - CORRECT ANSWERS-100x
means that you can change the focus (objective lens) of a microscope without having to
focus it every time - CORRECT ANSWERS-parfocal
how do you change the light reaching the ocular lens? - CORRECT ANSWERS-light
under the stage or the iris diaphragm
controls the amount of light that is passing through (the larger the hole the more light) -
CORRECT ANSWERS-iris diaphragm
single celled - CORRECT ANSWERS-protozoa
do you need oil immersion for eukaryotes? - CORRECT ANSWERS-no because
eukaryotes are so big you do not need to magnify them beyond 40x
gram positive rod - CORRECT ANSWERS-b. cereus
responsible for making beer - CORRECT ANSWERS-s. cerveisiae
why do you heat fix a specimen? - CORRECT ANSWERS-to attach the bacteria to the
slide so it doesn't wash off during the staining process
why do you use a wet mount? - CORRECT ANSWERS-the water helps to improve
clarity of the organism also if it was a live organism you would be able to see it move
why do we use simple staining techniques? - CORRECT ANSWERS-allows us to see
size, morphology, and arrangement
why do you air dry your slide? - CORRECT ANSWERS-it makes sure that the bacteria
is dry so when you heat it it doesn't change the shape of the bacteria
gram positive bacteria (cocci) - CORRECT ANSWERS-s. epidermidis
purple - CORRECT ANSWERS-gram positive
pink - CORRECT ANSWERS-gram negative
thick layer of peptidoglycn - CORRECT ANSWERS-gram positive
thin layer of peptidoglycan with an outer membrane that contains lipid a - CORRECT
ANSWERS-gram negative
