Revision Quiz on Production of Recombinant Products and Bioreactors

Module: Date:



Recombiant products
Revision of Recombiant Products Production




1 Ligase has what function in DNA? 6 What are the purification methods used in
downstream processing?
a) Repairs DNA by joining nucleotides in a 1)
DNA strand
b) Breaks DNA by cleaving disulfide bonds 2)
c) It doesn’t have an effect its neutral with DNA
3)
2 What characteristics must vectors have? 7 Where can gene of interest (GOI) be
sourced from?
a) Multiple cloning sites (MCS)
a) bacteria, yeast, fungi, plant cells, animal cells
b) Origin of DNA replication, multiple
b) bacteria, fungi, plant cells, animal cells
cloning sites and marker genes
c) bacteria and animal cells

c) circular, large enough to hold GOI
and an ORI (Origin of replication)
8 PCR is used for ....
3 Name the transformation methods used?

1)

2)

3)

4 What are the different types of bioreactors 9
Why are bioreactors used in production
of recombinant products

1)

2)

3)


5 What is the difference between batch and 10 Circle the technique associated with
fed batch? clarification

a) centrifugation
b)filtration
c) precipitation