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Summary Exploring Protein Detection: A Guide to Modern Assays

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PDF presentation that summarizes, protein assays and detection methods. Includes the basic steps of an ELISA and why they are required. Alongside types of detection (direct/indirect) as well as types of ELISA (direct, indirect, sandwich, competitive) and Immunohistochemistry uses

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Assays for P
Detecti

,Basic ELISA Steps

Coating of solid phase
with antibody and antigen
Block remaining binding
sites
Add antigen/antibody to
Use a s
be detected
detec
Wash out excess reagent amplificati

, An
Int
Antibodies de

Stronger Ab-A
of lower
Specificity of b
with serum pro
Balancing sen

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Institution: ATU
Course: AU858 (BIOL07013)

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Uploaded on: May 28, 2024
Number of pages: 26
Written in: 2023/2024
Type: SUMMARY

Subjects

gel electrophoresis
assay
elisa
antigens
antibodies
sandwich elisa
competitive elisa
direct detection
indirect detection
hrp
caoture antibodies
detection antibodies

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maggievarley Ulster University

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NFQ 7 in Biomedical/medical biotechnology Science NFQ 8 in medical Science Supplement notes and questions to help study along with answers to MCQs

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Summary Exploring Protein Detection: A Guide to Modern Assays

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