SBB Human Blood Groups Questions & Answers

SBB Human Blood Groups How would Ulex europaeus react with O, A, A2, B, A2B, A1B RBCs? - Answer- OA2BA2BA1A1B How could you distinguish serologically A1, A2 and A3 - Answer- Dolichos biflorus can will react with A1 and not A2. A3 yields mixed field reactions with anti-A and anti-A,B What is the correct order of type of RBCs to transfuse in an AB patient when AB RBCs are not available? Why? - Answer- A, B, O; the residual anti-B in A RBCs is less immunogenic than the residual anti-A in B RBCs What type of RBCs would you transfuse to a patient with anti-G - Answer- RBCs transfused must lack the D and C antigens Can you detect a weak D using Gel Technology? - Answer- No, because there is no washing phase Can an Rh positive father and Rh positive mother yield an Rh negative child - Answer- Yes, if both parents are heterozygous for the D gene. Inheritance is autosomal recessive What type of RBCs in regards to Rh phenotype would you transfuse in a patient with anti-f - Answer- RBCs should be negative for the c and e antigens How could you solve an ABO discrepancy where Anti-M was causing aberrant results in reverse grouping when the forward type is consistent with A blood group and reverse as O? - Answer- Treat A1 cells with ficin to denature the M antigen, repeat reverse grouping with ficin-treated A1 cells. Why do you see an anti-N like specificity in dialysis patients? - Answer- Formaldehyde induced antibody to 'N' due to formaldehyde used in dialysis procedures. A patient types as follows: anti-A=4+, Anti-B=1+, A1 Cells=2+, B cells=4+. The ABO discrepancy led to suspicion of a possible cold autoantibody based on patient histopry and diagnosis. RESt was used in an attept to adsorb the autoantobidy. The type was repeated with adsorbed plasma Anit-A=4+, Anti-B=0, A1 cells=0, B cells=0. What could be the cause of this discrepancy? - Answer- Anti-B was adsorbed out with RESt. What enzyme may be useful in distinguishing anti-Ge2? - Answer- papain The beta 1-3 linkage between galactose and N-acetylglucosamine is found in? - Answer- secretions