MOLECULAR BIOLOGY OF THE CELL, SIXTH EDITION
CHAPTER 18: CELL DEATH
© Garland Science 2015
1. Which of the following morphological changes is NOT typically seen in a cell that is
undergoing apoptosis?
A. The cell rounds up.
B. The nuclear envelope disassembles.
C. The cell swells.
D. Large cells break up into membrane-enclosed fragments.
E. The nuclear chromatin breaks into fragments.
m
er as
co
1. Indicate true (T) and false (F) statements below regarding programmed cell death by
eH w
apoptosis. Your answer would be a four-letter string composed of letters T and F only, e.g. TTTF.
o.
( ) Apoptosis is the final fate of almost all cells in an adult animal.
rs e
ou urc
( ) Even perfectly healthy cells may undergo apoptosis.
( ) DNA damage that cannot be repaired inhibits apoptotic pathways.
( ) Apoptosis is the main form of programmed cell death in plant cells as well as in
o
animal cells.
aC s
vi y re
1. Initiator and executioner caspases share all of the following features EXCEPT that ...
A. they are cysteine proteases (they have a cysteine residue at their active site).
ed d
B. their inactive form is a monomer.
ar stu
C. they undergo cleavage during activation.
D. their active form is a dimer.
E. they are inhibited by IAPs.
sh is
Th
1. v-FLIPs are viral proteins that were first identified as modulators of apoptosis; they
contain two death effector domains, which are also found in some initiator caspases such as
procaspase-8. These v-FLIP proteins can be recruited to the DISC through the binding of the
death effector domain to similar domains in the adaptor proteins, but are otherwise catalytically
inactive. What do you think is the effect of v-FLIP expression in the host cell?
A. It promotes apoptosis mainly via the extrinsic pathway.
B. It inhibits the extrinsic pathway of apoptosis.
C. It activates only the mitochondrial pathway of apoptosis.
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, D. It inhibits the intrinsic pathway of apoptosis.
E. It enhances the caspase cascades in both the intrinsic and extrinsic pathways.
1. Fill in the blank: In the intrinsic pathway of apoptosis, a protein called ... is released from
the mitochondria into the cytosol and binds to the adaptor protein Apaf1, causing it to
oligomerize into a wheel-like assembly called an apoptosome, which then recruits initiator
caspase-9 proteins.
1. The Bcl2 family is comprised of anti-apoptotic (A), BH3-only (B), and effector (E)
proteins. In the following diagram representing the regulation of the intrinsic pathway of
apoptosis, what class of activated Bcl2 family proteins (A, B, or E) corresponds to boxes 1 to 3,
m
respectively? Your answer would be a three-letter string composed of letters A, B, and E only,
er as
e.g. ABE.
co
eH w
Released mitochondrial
1 2 3
o.
intermembrane
rs e
ou urc
Initiator
o
!
aC s
vi y re
1. Which of the following proteins activates the mitochondrial pathway of apoptosis?
A. The tumor suppressor protein p53, when activated in response to extensive DNA
ed d
damage.
ar stu
B. The BH3-only protein Bid, when cleaved by the initiator caspase-8 (from the
extrinsic pathway).
sh is
C. The anti-IAP protein Omi, when activated by dephosphorylation.
Th
D. The BH3-only protein Bad, when activated by dephosphorylation.
E. All of the above.
1. Indicate whether each of the following mutations would likely promote (P) or inhibit (I)
apoptosis in cells harboring the mutation(s). Your answer would be a four-letter string composed
of letters P and I only, e.g. PPPI.
( ) Mutations in the pro-apoptotic effector Bcl2 family proteins Bax and Bak that prevent
their association with the outer mitochondrial membrane.
This study source was downloaded by 100000830124955 from CourseHero.com on 04-26-2022 09:08:04 GMT -05:00
https://www.coursehero.com/file/19104397/MBoC6QuestionBank-Ch18/
https://www.coursehero.com/file/39176186/pdf-8pdf/
CHAPTER 18: CELL DEATH
© Garland Science 2015
1. Which of the following morphological changes is NOT typically seen in a cell that is
undergoing apoptosis?
A. The cell rounds up.
B. The nuclear envelope disassembles.
C. The cell swells.
D. Large cells break up into membrane-enclosed fragments.
E. The nuclear chromatin breaks into fragments.
m
er as
co
1. Indicate true (T) and false (F) statements below regarding programmed cell death by
eH w
apoptosis. Your answer would be a four-letter string composed of letters T and F only, e.g. TTTF.
o.
( ) Apoptosis is the final fate of almost all cells in an adult animal.
rs e
ou urc
( ) Even perfectly healthy cells may undergo apoptosis.
( ) DNA damage that cannot be repaired inhibits apoptotic pathways.
( ) Apoptosis is the main form of programmed cell death in plant cells as well as in
o
animal cells.
aC s
vi y re
1. Initiator and executioner caspases share all of the following features EXCEPT that ...
A. they are cysteine proteases (they have a cysteine residue at their active site).
ed d
B. their inactive form is a monomer.
ar stu
C. they undergo cleavage during activation.
D. their active form is a dimer.
E. they are inhibited by IAPs.
sh is
Th
1. v-FLIPs are viral proteins that were first identified as modulators of apoptosis; they
contain two death effector domains, which are also found in some initiator caspases such as
procaspase-8. These v-FLIP proteins can be recruited to the DISC through the binding of the
death effector domain to similar domains in the adaptor proteins, but are otherwise catalytically
inactive. What do you think is the effect of v-FLIP expression in the host cell?
A. It promotes apoptosis mainly via the extrinsic pathway.
B. It inhibits the extrinsic pathway of apoptosis.
C. It activates only the mitochondrial pathway of apoptosis.
This study source was downloaded by 100000830124955 from CourseHero.com on 04-26-2022 09:08:04 GMT -05:00
https://www.coursehero.com/file/19104397/MBoC6QuestionBank-Ch18/
https://www.coursehero.com/file/39176186/pdf-8pdf/
, D. It inhibits the intrinsic pathway of apoptosis.
E. It enhances the caspase cascades in both the intrinsic and extrinsic pathways.
1. Fill in the blank: In the intrinsic pathway of apoptosis, a protein called ... is released from
the mitochondria into the cytosol and binds to the adaptor protein Apaf1, causing it to
oligomerize into a wheel-like assembly called an apoptosome, which then recruits initiator
caspase-9 proteins.
1. The Bcl2 family is comprised of anti-apoptotic (A), BH3-only (B), and effector (E)
proteins. In the following diagram representing the regulation of the intrinsic pathway of
apoptosis, what class of activated Bcl2 family proteins (A, B, or E) corresponds to boxes 1 to 3,
m
respectively? Your answer would be a three-letter string composed of letters A, B, and E only,
er as
e.g. ABE.
co
eH w
Released mitochondrial
1 2 3
o.
intermembrane
rs e
ou urc
Initiator
o
!
aC s
vi y re
1. Which of the following proteins activates the mitochondrial pathway of apoptosis?
A. The tumor suppressor protein p53, when activated in response to extensive DNA
ed d
damage.
ar stu
B. The BH3-only protein Bid, when cleaved by the initiator caspase-8 (from the
extrinsic pathway).
sh is
C. The anti-IAP protein Omi, when activated by dephosphorylation.
Th
D. The BH3-only protein Bad, when activated by dephosphorylation.
E. All of the above.
1. Indicate whether each of the following mutations would likely promote (P) or inhibit (I)
apoptosis in cells harboring the mutation(s). Your answer would be a four-letter string composed
of letters P and I only, e.g. PPPI.
( ) Mutations in the pro-apoptotic effector Bcl2 family proteins Bax and Bak that prevent
their association with the outer mitochondrial membrane.
This study source was downloaded by 100000830124955 from CourseHero.com on 04-26-2022 09:08:04 GMT -05:00
https://www.coursehero.com/file/19104397/MBoC6QuestionBank-Ch18/
https://www.coursehero.com/file/39176186/pdf-8pdf/
