Isolation and Purification of
Virus
It is difficult to isolate virus ,
as
compared to that ofbacteria ,
because unlike bacteria ,
viruses
living host cell Infectedhost cells (eukaryotic andprokaryotic) be cultured
require a .
can
and be harvested as source of virus
grown
and then the
growth medium
,
can a
Generally cell cultures embryohatedeggs and small laboratory animals
,
used for are
the isolation of influenzae and viruses
paramyxo .
Tor cell cultures tissue first dissociatedinto small
primary fragments ,
are
pieces with the
scissors and addition of
help of
trypsin .
The washed
cell
suspension is then
couple of times with minimal essential media and
seededinto a flat-bottomed
glass or plastic container after re suspending it with a
suitable and fetalserum
liquid medium .
the cell is
kept in incubatorat 37°C for 24 to 48hours
depending on the cell type
liquidmedium can be host
virions in the
separated from the ^
cells
by either
centrifugation
'
or filtration .
the solution that
Filters can
physically remove
anything present in is
larger than the virions
,
the viruses can be collected in the filtrate .
Centrifugation :
is convenient cells and
low -
speed centrifugation :
a simple way
to
purify viruses .
and the
large cellular debris are
pelleted , suspended visions in the supernatant can be
Virus
It is difficult to isolate virus ,
as
compared to that ofbacteria ,
because unlike bacteria ,
viruses
living host cell Infectedhost cells (eukaryotic andprokaryotic) be cultured
require a .
can
and be harvested as source of virus
grown
and then the
growth medium
,
can a
Generally cell cultures embryohatedeggs and small laboratory animals
,
used for are
the isolation of influenzae and viruses
paramyxo .
Tor cell cultures tissue first dissociatedinto small
primary fragments ,
are
pieces with the
scissors and addition of
help of
trypsin .
The washed
cell
suspension is then
couple of times with minimal essential media and
seededinto a flat-bottomed
glass or plastic container after re suspending it with a
suitable and fetalserum
liquid medium .
the cell is
kept in incubatorat 37°C for 24 to 48hours
depending on the cell type
liquidmedium can be host
virions in the
separated from the ^
cells
by either
centrifugation
'
or filtration .
the solution that
Filters can
physically remove
anything present in is
larger than the virions
,
the viruses can be collected in the filtrate .
Centrifugation :
is convenient cells and
low -
speed centrifugation :
a simple way
to
purify viruses .
and the
large cellular debris are
pelleted , suspended visions in the supernatant can be
