PORTAGE LEARNING BIOD 171 MODULE 3 EXAM QUESTIONS WITH COMPLETE SOLUTIONS VERIFIED

PORTAGE LEARNING BIOD 171 MODULE 3 EXAM QUESTIONS WITH COMPLETE SOLUTIONS VERIFIED Does phase contrast microscopy require staining? No Simple staining is used mostly for... A quick observance Simple staining uses __________ dye. Positively charged Acid fast stain is used for... bacteria resistant to traditional staining; mostly for mycobacterium tuberculosis Resolution distance between two objects at which the objects can still be seen as separate Contrast difference in light absorbance between 2 areas Dark field microscopy light reflects light off of specimen at an angle Can you see intracellular structures with a dark field microscope? No What microscope can capture in 3-D? Confocal What are the two types of electron microscopes? transmission electron microscope (TEM) and scanning electron microscope (SEM) What is the difference between TEM and SEM? TEM passes through the sample, SEM reflects off the surface Gram staining was developed by Hans Christian Gram in 1884 Gram-positive bacteria shows that there is a thick peptidoglycan cell wall What color will gram positive bacteria stain? purple What color will gram negative bacteria stain? pink A gram negative bacteria has a... think peptidoglycan cell wall Differential Stain a stain that distinguishes objects on the basis of reactions to the staining procedure What are the two types of cell fixation? Heat fixation and Chemical fixation What does Giesma detect? Blood parasites What is stained during negative staining? Everything but the microorganism What color does Giesma stain? What type of cell?