Exam 2 Review
3:09 PM
Quiz Questions
Chapter 7:
• Which enzym e forms the final phosphodiester bond between Okazaki fragments?
○ Ligase
• A replication bubble is depicted below. Given what is shown, in what direction do you infer the top blue
strand runs?
3'<-5'
• The double stranded DNA below is amplified by PCR using the primers shown below. How many double-
stranded DNA products will the researchers have after 3 rounds of PCR?
23
(2n, n = number of rounds of PCR. i.e. 30 rounds = 230)
• Which statement is NOT true about Origins of Replication?
○ OOR are where the replication bubble forms that advance bidirectionally
○ Multiple OOR in a eukaryote linear chromosome
○ OOR are recognized by proteins to start replication
○ OOR have particular sequences
○ There are multiple OOR in a prokaryote circular chromosome
• Below is the double stranded DNA sequence. How many hydrogen bonds keep these two strands
together?
○ 22 hydrogen bonds
5'-CTTAGCATG-3'
3'-GAATCGTAC-5'
CG pairs contain 3 hydrogen bonds
AT pairs contain 2 hydrogen bonds
• A DNA double helix molecule that is 100 base pairs long has a GC content of 30%. How many hydrogen
Genetics Page 1
, bonds are present in this double helix? 230 hydrogen bonds
Genetics Page 2
3:09 PM
Quiz Questions
Chapter 7:
• Which enzym e forms the final phosphodiester bond between Okazaki fragments?
○ Ligase
• A replication bubble is depicted below. Given what is shown, in what direction do you infer the top blue
strand runs?
3'<-5'
• The double stranded DNA below is amplified by PCR using the primers shown below. How many double-
stranded DNA products will the researchers have after 3 rounds of PCR?
23
(2n, n = number of rounds of PCR. i.e. 30 rounds = 230)
• Which statement is NOT true about Origins of Replication?
○ OOR are where the replication bubble forms that advance bidirectionally
○ Multiple OOR in a eukaryote linear chromosome
○ OOR are recognized by proteins to start replication
○ OOR have particular sequences
○ There are multiple OOR in a prokaryote circular chromosome
• Below is the double stranded DNA sequence. How many hydrogen bonds keep these two strands
together?
○ 22 hydrogen bonds
5'-CTTAGCATG-3'
3'-GAATCGTAC-5'
CG pairs contain 3 hydrogen bonds
AT pairs contain 2 hydrogen bonds
• A DNA double helix molecule that is 100 base pairs long has a GC content of 30%. How many hydrogen
Genetics Page 1
, bonds are present in this double helix? 230 hydrogen bonds
Genetics Page 2
