BMSC 210 Midterm 2 Quizzes | 100% Correct
Answers | Verified | Latest 2024 Version
what can restore a frameshift mutation? - ✔✔another frameshift, microdeletion, and microinsertion
A mutation in a particular gene that results in the premature halting of transcription (and, consequently,
protein translation) is a___________ mutation. - ✔✔nonsense
Consider a mutation in which the change is from UAC to UAU. Both codons specify the amino acid
tyrosine. Which type of point mutation is this? - ✔✔Silent mutation
A ʺpoint mutationʺ refers to mutations involving - ✔✔a substitution, deletion, or addition of one base-
pair.
Nutritionally defective mutants can be detected by the technique of - ✔✔replica plating
Which method would be the MOST powerful approach to isolating a single mutant from a population of
billions? - ✔✔selection for a gain of function
(like finding a needle in a haystack)
A mutant that has a nutritional requirement for growth is an example of a(n) - ✔✔auxotroph
A plasmid may - ✔✔replicate independently of the chromosome, integrate into the chromosome, or be
transferred cell-to-cell during conjugation.
The uptake of free DNA from the environment is referred to as ________, while the transfer of DNA with
cell-to-cell contact is known as ________. - ✔✔transformation/conjugation
The CRISPR system - ✔✔recognizes foreign DNA sequences that have previously entered the cell and
directs the Cas proteins to destroy them.
, Lysogeny probably carries a strong selective advantage for the host cell because it - ✔✔confers
resistance to infection by viruses of the same type.
F+ strains of Escherichia coli - ✔✔have the F factor as a plasmid
When DNA is transferred into a prokaryotic cell, it may. - ✔✔be degraded by enzymes, replicate
independent of the host chromosome, or recombine with the host chromosome.
Consider the following experiment. First, large populations of two mutant strains of Escherichia coli are
mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45
colonies grew. Which of the following may explain this result?
A) The colonies may be due to back mutation (reversion).
B) The colonies may be due to recombination. - ✔✔Either A or B is possible
The F (fertility) plasmid contains a set of genes that encode for the ________ proteins that are essential
in conjugative transfer of DNA. - ✔✔pili
During electrophoresis, DNA - ✔✔is separated by charge
Which is NOT true about PCR?
A. It uses DNA polymerases from psychrophilic microbes
B. It is used for making DNA for cloning and DNA sequencing
C. It amplifies DNA sequences
D. It uses oligonucleotide primers that are complementary to the flanking regions of target DNA - ✔✔A.
It uses DNA polymerases from psychrophilic microbes
RT-PCR makes _______ from a(n) _______ template. - ✔✔cDNA; mRNA
If you wanted to compare the relative abundance of mRNA transcribed for a specific gene by two
different strains of bacteria, you would perform - ✔✔a Northern blot
Answers | Verified | Latest 2024 Version
what can restore a frameshift mutation? - ✔✔another frameshift, microdeletion, and microinsertion
A mutation in a particular gene that results in the premature halting of transcription (and, consequently,
protein translation) is a___________ mutation. - ✔✔nonsense
Consider a mutation in which the change is from UAC to UAU. Both codons specify the amino acid
tyrosine. Which type of point mutation is this? - ✔✔Silent mutation
A ʺpoint mutationʺ refers to mutations involving - ✔✔a substitution, deletion, or addition of one base-
pair.
Nutritionally defective mutants can be detected by the technique of - ✔✔replica plating
Which method would be the MOST powerful approach to isolating a single mutant from a population of
billions? - ✔✔selection for a gain of function
(like finding a needle in a haystack)
A mutant that has a nutritional requirement for growth is an example of a(n) - ✔✔auxotroph
A plasmid may - ✔✔replicate independently of the chromosome, integrate into the chromosome, or be
transferred cell-to-cell during conjugation.
The uptake of free DNA from the environment is referred to as ________, while the transfer of DNA with
cell-to-cell contact is known as ________. - ✔✔transformation/conjugation
The CRISPR system - ✔✔recognizes foreign DNA sequences that have previously entered the cell and
directs the Cas proteins to destroy them.
, Lysogeny probably carries a strong selective advantage for the host cell because it - ✔✔confers
resistance to infection by viruses of the same type.
F+ strains of Escherichia coli - ✔✔have the F factor as a plasmid
When DNA is transferred into a prokaryotic cell, it may. - ✔✔be degraded by enzymes, replicate
independent of the host chromosome, or recombine with the host chromosome.
Consider the following experiment. First, large populations of two mutant strains of Escherichia coli are
mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45
colonies grew. Which of the following may explain this result?
A) The colonies may be due to back mutation (reversion).
B) The colonies may be due to recombination. - ✔✔Either A or B is possible
The F (fertility) plasmid contains a set of genes that encode for the ________ proteins that are essential
in conjugative transfer of DNA. - ✔✔pili
During electrophoresis, DNA - ✔✔is separated by charge
Which is NOT true about PCR?
A. It uses DNA polymerases from psychrophilic microbes
B. It is used for making DNA for cloning and DNA sequencing
C. It amplifies DNA sequences
D. It uses oligonucleotide primers that are complementary to the flanking regions of target DNA - ✔✔A.
It uses DNA polymerases from psychrophilic microbes
RT-PCR makes _______ from a(n) _______ template. - ✔✔cDNA; mRNA
If you wanted to compare the relative abundance of mRNA transcribed for a specific gene by two
different strains of bacteria, you would perform - ✔✔a Northern blot
