BIOCHEM ENGINEERING:
BIOSEPARATIONS QUESTIONS WITH
COMPLETE ANSWERS { GRADED A+}
depth filtration - ✔✔different layers of filtration as it goes deeper,
particles retained within its structure not on surface
surface filtration - ✔✔fixed pore size that stops larger microorganisms
on the surface
continuous centrifugation advantages - ✔✔1. high efficiency in
separation from supernatant
2. continuous
3. no clogging
continuous centrifugation disadvantages - ✔✔1. high maintenance
, 2. aerosol formation
3. high cost
products that need cell disruption - ✔✔1. lipids and carbs for vaccines
like meningitis
2. nucleic acids (RNA therapeutics)
3. virus like particles (HepB)
4. recombinant proteins (interferons)
high pressure homogenizer - ✔✔pressure change and high shear
through orifice breaks cells; very effective but can result in high temps
high speed ball mix - ✔✔blender with steel balls that takes a long time
and a lot of energy
enzymatic lysis - ✔✔1. degrading enzymes
2. detergents solubilize lipid of membrane
3. for industrial chemical production
BIOSEPARATIONS QUESTIONS WITH
COMPLETE ANSWERS { GRADED A+}
depth filtration - ✔✔different layers of filtration as it goes deeper,
particles retained within its structure not on surface
surface filtration - ✔✔fixed pore size that stops larger microorganisms
on the surface
continuous centrifugation advantages - ✔✔1. high efficiency in
separation from supernatant
2. continuous
3. no clogging
continuous centrifugation disadvantages - ✔✔1. high maintenance
, 2. aerosol formation
3. high cost
products that need cell disruption - ✔✔1. lipids and carbs for vaccines
like meningitis
2. nucleic acids (RNA therapeutics)
3. virus like particles (HepB)
4. recombinant proteins (interferons)
high pressure homogenizer - ✔✔pressure change and high shear
through orifice breaks cells; very effective but can result in high temps
high speed ball mix - ✔✔blender with steel balls that takes a long time
and a lot of energy
enzymatic lysis - ✔✔1. degrading enzymes
2. detergents solubilize lipid of membrane
3. for industrial chemical production
